Honeysuckle Alcohol Extract Of The Role Of Enterococcus Faecalis In Root Canals In Vitro Studies

Object:At present,research of domestic and foreign reported that the pathogenicity of E. faecalis in pulposis and periapical disease. In this study,by compared ethanol extracts of honeysuckle with sodium hypochlorite solution on Inhibition in E.faecalis to Verify it's antimicrobial activity in E.faecalis .And further study ethanol extracts of honeysuckle against E.faecalis biofilm and and adhesion ability of E.faecalis In the early,theoretical basis for use of honeysuckle active ingredients against E.faecalis infection in the future.Method: plate-hole diffusion assay and direct contact test was used to verify E.faecalis on the sensitivity of ethanol extract of honeysuckle, the direct contact test was determined that the minimal inhibitory concentration(MIC) of ethanol extracts of honeysuckle on E.faecalis invitro using MTT assay assisted,verify the accuracy of this method with plate cultivation;Established E.faecalis mono-biofilm model,in vitro. After processed with ethanol extracts of honeysuckle,reckon the Inhibition rate of ethanol extracts of honeysuckle to E.faecalis mono-biofilm ,MTT assay was used.Fluorescent stained with propidium iodide(PI)and examined through used of CLSM,observed the effect of drugs on the E.faecalis mono-biofilm.adherent materials of biofilm pre-processed with 200mg/ml ethanol extracts of honeysuckle then established E.faecalis mono-biofilm model,Samples to be tested with metabolic activity using MTT assay,and examined through use of CLSM to observed the adhesion of E.faecalis on the adherent materials.Result:The 800mg/ml ethanol extracts of honeysuckle (22.60±0.95)was the most effective in the plate-hole diffusion assay ,it had no significant difference with 5.25% sodium hypochlorite solution(20.70±1.11)(P>0.05),The results of MIC for ethanol extracts of honeysuckle was 200mg/ml in direct contact test. The MIC result determined by plate cultivation was similar to the result by MTT assay assisted.Inhibitory effect of ethanol extracts of honeysuckle to E.faecalis mono-biofilm increased with the drug concentration.800mg/ml ethanol extracts of honeysuckle acquired maximum inhibition rate(81.16%),and 25mg/ml ethanol extracts of honeysuckle acquired minimum inhibition rate(41.76%).The results showed significant statistical difference (P<0.05)between the experimental group and the negative control group.The results showed ethanol extracts of honeysuckle could decrease the vitality of E faecalis in the biofilm significantly.The effect of ethanol extracts of honeysuckle was obvious to the bacterial in E.faecalis mono-biofilm surface,inner bacterial couldn't be completely inactivated even higher concentrations ethanol extracts of honeysuckle was used.Adhesion ability of E.faecalis was significantly inhibited after adherent materials be pre-processed.The MTT result showed significant statistical difference (P<0.05) to metabolic activity between the experimental group and the control group.Conclusion:1. The ethanol extracts of honeysuckle showed satisfactory antibacterial activity against E.faecalis.2. The effect of ethanol extracts of honeysuckle was obvious to Inhibited E.faecalis mono-biofilm,effect especially to the bacterial in E.faecalis mono-biofilm surface,but Even with a higher concentration of physic liquor couldn't completely killed the bacteria in biofilms.3. Ethanol extracts of honeysuckle could reduce the adhesion ability of E. faecalis. The results indicates that it could prevent the formation of E.faecalis mono-biofilm.