| The phenomena of mixing the false with the genuine and selling the shoddy medicines, which is often taken plase, has badly restricted the security of employing CTMs and the staibilization of the curative effects, and it has also restricted the developments of the research and application of CTMs. In order to ensure the employ security and stabilization of the curative effect of the CTMs, and providing the entrance of CTMs into the international medicine market with the quality guarantee, how to identify and evaluate CTMs scientifically and quickly is one of the challenging problems in analytical chemistry. Nonlinear chemical fingerprint is a new method for CTMs identification and evaluation in view of nonlinear chemistry. Comparing to chromatographic fingerprint and spectrum fingerprint, it is no need to pretreat such as separation and purification, and suitable for using in the throng token of chemical components in various phases and prepared forms of CTMs, with characteristics of fast analysis and low cost. In this thesis, the similarity, accuracy and application were researched completely and systematically, and the nonlinear chemical fingerprint intelligent analyzer was designed. The main contents of the thesis are as follows.1. Basic theoretical study on nonlinear chemical fingerprint Similarity calculation method for nonlinear chemical fingerprint was proposed, and the accuracy of TCM identification was investigated. The determination principle for the fingerprint was put forward, and 18 TCMs nonlinear chemical fingerprints was obtained according to the principle. The similarities of NLC fingerprints of Radix Glycyrrhizae from different habitats and the other traditional Chinese medicines (TCMs) of different kinds were calculated, using similarity system theory and other three similarity calculating methods(Euclidean distance, correlation coefficient, included angle cosine) which are the main calculation methods of fingerprints at present. The computational results clearly demonstrated that the proposed method was a powerful approach which could faithfully reflect the differences between samples, and could be used as quantitative criteria of similarity degree of NLC fingerprints of TCMs. The accuracy calculation method for traditional Chinese medicines identification using nonlinear chemical fingerprints was derived based on B-Z oscillating mechanism and probability theory, and to verify its accuracy, experiment was conducted for identifying different traditional Chinese medicines. Therefore, combining similarity calculation with the intuitionistic features of the fingerprints, the method can be used to distinguish and evaluate TCMs quickly, simply and truly.2. Application study on nonlinear chemical fingerprint The principle of nonlinear chemical fingerprint based on nonequilibrium state was introduced, and combining which to similarity calculation, Glycyrrhizae was identified and evaluated. According to the linear relationship between the holistic content of TCM components (or determination dosage) and the inductive time of TCM non-linear electrochemical fingerprints, relative global contents of active components of glycyrrhiza from four different producing areas was calculated, and their equivalent relationship was established. It provides a new method for discrimination and evaluation of glycyrrhiza, and dosage reference for clinical medication and compatibility, which is help to security and stability of TCMs medication.The nonlinear chemical fingerprint based on equilibrium state was introduced, and its principle, characteristic and application were studied taken the " H++Mn2+ +BrO3-+acetone" for example. Also, advantages and disadvantages of the two methods were compared.3. Design and development of nonlinear chemical fingerprint intelligent analyzerThe analyzer was designed and assembled, with a steady temperature device whose temperature variation range was±0.1℃,a stirring motor whose control precision was 1%, and a measurement system whose signal processing error was below 0.5%.Date collection software and date procession software were compiled, which can choose communication port, data sampling interval, sampling points and other parameters, and realize high-speed data acquisition and signal visualization. It also can process the fingerprint data such as plotting, smoothing and so on, calculate quantitative parameters, similarity automatically, and do pattern analysis as well. Therefore, we developed a complete platform from data collection to data procession, which srongly promote the application and development of nonlinear chemical fingerprint. |
PDF Full Text Request