| The dissertation is composed of 2 parts. In the first part there are three review articles. The first review articles we discussed the research of vomiting animal model, including its selecting animal, methods lead to vomiting and evaluation its results. The second review articles we reviewed the Chinese medical researches on curing chemotherapy induced nausea and vomiting (CINV), including its conception, classification, ancient studies, pathogenesis and treatment. The third review articles we systematically reviewed the mechanism of vomiting chemotherapy and experimental study of traditional Chinese medicine.Experimental section includes the following:Objective:To observe the pre-ventive and therapeutic effect of Medicine Application on Navel on chemother apy induced nausea and vomiting (CINV) and to study its action mechanism.Methods:1. Select Umbilical Sticking Plaster acute toxicity observed in mi ce skin reaction.2. Intraperitoneal injection of cisplatin pigeon animal models, use 60 pigeons in accordance with the randomized block design was divided i nto the high-dose, middle dose and low dose group, positive medicine group, model group, control group of six groups.3. Observers pigeon vomiting mode-1 latency and vomiting after single injection of cisplatin daily for 5 consecutive hours for 3 consecutive days, frequency of vomiting.4. The level of pigeon plasma motilin, vasoactive intestinal peptide and gastrin,5-HT, SP and DA in cerebral tissue or in Intestine tissue were measured with radioimmunoassgy.Results:1.Acute toxicity test in Mice skin:Compared to observation group, no significantly diversification was in experimental group, including swelling of mouse skin, damage, eye conjunctiva, mucous membranes and breathing, eatin g, limb activities were normal, usual food intake with.2. Latency of vomiting of TCM group was significantly longer than model group (model group:3.03h, Medicines:3.44-3.62 h), P<0.05; Compared TCM groups to positive group, vo miting latency showed no significantly diversification (positive group 3.75h), freq uency of vomiting of TCM medium dose Group and large dose group compare d to model group (model group:7.2 times, the Chinese Medicines line 2.8-5.9 times) was significantly reduced, P<0.05; All TCM group showed significant dose-effect relationship, Chinese high-dose group was less than the frequency o f vomiting to positive group (positive group:6.0 times), P<0.01.3.Chinese gr oup (large & medium dose group) was significantly higher than plasma MTL model group (P<0.05), Positive group compared to model group was no signif icantly difference; GAS of TCM groups was significantly lower than model gr oup (P<0.05), Positive group compared to model group was no significantly d ifference; Plasma VIP was significantly higher in TCM groups than the model group and control group (P<0.05), positive group and model groupwas no sig nificant difference.4. Neurotransmitter changes in peripheral blood:P'substance was significantly higher in Chinese high-dose group than in group (P<0.05),5-HT levels decreased significantly in all the Chinese groups than in model gro up, P<0.05; DA levels in all the Chinese group increased significantly than in model group, (P<0.05), P substance, DA,5-HT in positive group was no sig nificantly changed compared with the model.5. Neurotransmitter changes Intesti nal tissue:P substance was significantly lower in all the TCM groups and posi tive group than in model group (P<0.05), traditional Chinese medicine group and the positive group were no significant difference; 5-HT was significantly h igher in Chinese high-dose and medium-dose group than in model group, (P< 0.05); the DA in herb medium-dose group than in model group was significant ly lower, P<0.05.6.6. Neurotransmitter changes in brain; P substance in Chin ese high-dose group and positive group were significantly lower than in model group, P<0.05; 5-HT in all TCM group and the positive group did not chan ge significantly; medium-dose group and low-dose group was significantly lowe r than in model groups, P<0.05, DA in positive group were significantly high er than in the model groups.Conclusion:1. Umbilical Sticking Plaster on skin is safety without stimula tion, non-toxic.2. Umbilical Compressing cream can help prevent cisplatin-indu ced nausea and vomiting in pigeons, mainly in the extended incubation period of vomiting, decreased frequency of vomiting, extended incubation period of vo miting in positive group was better than medicine group, may be related to gra nisetron absorbed faster. Frequency of vomiting in TCM high dose group was better than positive group.3. peripheral mechanisms for nausea and vomiting o f Umbilical Sticking Plaster:It possible increased plasma MTL, decreased GAS levels, increased gastrointestinal motility, decreased gastric acid secretion and pepsin secretion and thus play an antiemetic effect; It may antagonize peripher al NK1-receptor and DA-receptors, lower 5-HT levels in blood; It may reduce the intestinal tissue P substance, DA, antagonistic antiemetic 5-HT receptors pla y a role. Umbilical Sticking Plaster central mechanism of nausea and vomiting: P substance may reduce in the brain substance, DA antiemetic, central 5-HT r eceptor antagonist antiemetic play a role.4. Umbilical Sticking Plaster increase in appetite may because of increasing plasma MTL, lowering GAS.5. Umbili cal Sticking Plaster to treat delayed vomiting may due to reduce the peripheral and the central P-substance. |
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