| Shock lung (acute lung injury, ALI) is the main damaged organ in earlv septic shock In recent years, practitioners of Chinese medicine with modern medicine to the pathogenesis of ALI,which was useful to explore and put forward to a number of governance, According to the rule, which were Although experiment of research has made some effect, but the results is poor. The experiment is based on acute lung injury in the pathogenesis of virtual real evil. put forward ideas Yiqihuoxue detoxification, application LPS rat model of endotoxic shock and lung injury to observe the affect of the Qi and blood detoxification injection Shengmaiyin to shock lung.ObjectiveTo observe with Yiqihuoxue detoxification injection Shengmaiyin shock lung injury, and the possible role of links.MethodsGrope:SD rats were randomly divided into 4 groups, namely sham operation group, model group, Sheng mai yin injection group (Sheng mai yin group), dexamethasone group 24, each further divided into shock after lh,2h,3h,6h,4 phase, each phase to ensure that at least six rats successfully.Model:Copy intravenous LPS lung injury model of endotoxin shock and sham group received saline 20ml/kg, model group were given LPS 8mg/kg, dexamethasone group received LPS 8mg/kg＋dexamethasone 5mg/kg, group Shengmaiyin to LPS 8mg/kg＋Sheng mai yin injection 10 ml/kg, the treatment group was replicated after 10min of endotoxin shock model to the treatment of drug infusion in each group Liangjun control 20ml/kg. MAP from the baseline to 25%-30% lower, as in acute endotoxin shock model of success.Drawn:Select the middle left lung, the ice bath with 0.9% saline homogenate,4℃3500 r/min centrifugation 10 min, the supernatant prepared to take a 10% homogenate,-70℃preservation tested. Take the right middle lung tissue, lung tissue water content is about 100mg, the remaining part of the right lung in the home 10% neutral formalin fixed, making morphological specimens.Index:In general, mean arterial pressure, mortality, lung water content and morphology, chemical determination of the lung tissue SOD activity and MDA content, chemical determination of the lung tissue NO content and iNOS activity, RT-PCR to detect lung NOS1mRNA and FGF2mRNA expressionStatistics:Data were analyzed using SAS 8.2 software, the first test of homogeneity of variance, homogeneity of variance analysis of variance directly; variance parameters replace missing after the test of homogeneity of variance, homogeneity of variance by analysis of variance. Results indicated that differences between groups using analysis of variance (ANOVA/LSD), with P<0.05 significant difference as the standard.Results:General:Sham group animals generally in good condition; model animals after endotoxin injection, shortness of breath, lips Cyanosis, hypothermia, limbs Jueleng, chills; drugs after the intervention, significantly reduced shortness of breath, with malaise,Diarrhea, the performance of vertical hair.General indicators:①6h model mortality 83.3%, dexamethasone group and lower mortality than Shengmaiyin LPS model group (P<0.05), between the two groups on mortality was no significant difference (P> 0.05);②shock 6h, the model group than the sham group, lung water content significantly increased lung water content in the treated group was significantly lower than the model group, there was significant statistical difference (P<0.01), dexamethasone group and the group Shengmaiyin were no significant differences (p> 0.05);③sham group no significant changes in MAP Animals. Moaei animals after endotoxin injection, MAP decreased rapidly,30 min which has been picked up in the 90-120min gradually slow down, until death. Treatment group decreased blood pressure is reduced significantly (p<0.05). Shengmaiyin group and dexamethasone group, there was no significant difference (p> 0.05);④sham group, lung tissue was normal; model group shock 1h, found that many small blood vessels to dilate lung congestion, shock 6h, the majority of animal lung hemorrhage, thrombosis more common small; in the dexamethasone group structure of lung tissue damage does not show significant changes. Most animals are small blood vessels to dilate congestion; have three focal atelectasis animals there, two local small kitchen-like lung hemorrhage,13 lung cells together to form lymphoid follicles; Shengmaiyin rats (24 only) show that damage the lung tissue structure changes significantly reduced.24 rats was still found in many small blood vessels to dilate lung congestion.Indicator of oxygen free radical:①compared with the sham group, model group were significantly lower SOD activity (P<0.01); with the model group and dexamethasone group Shengmaiyin group SOD activity was significantly increased (P<0.01); Modified Shengmai group and dexamethasone group drinking between the two groups were significantly different (P<0.05),②compared with the sham group, model group, the lung tissue MDA levels were significantly higher (P<0.01); with model group, Shengmaiyin group and dexamethasone group, lung MDA was significantly lower (P<0.01, P<0.05); Shengmaiyin group and dexamethasone group there was significant difference (P<0.05).NO radical:Compared with the sham group, model group, the lung tissue NO concentrations and iNOS activity was significantly higher (P<0.01), application Shengmaiyin intervention injection or after treatment with dexamethasone, dexamethasone group and Shengmaiyin Injection lung tissue NO concentration and iNOS activity was significantly lower than the model (P<0.01, P<0.05), Shengmaiyin group and dexamethasone group there was significant difference (P<0.05).RT-PCR:Occurrence and development of endotoxin shock, the expression of NOS1 mRNA in the lung and shock shock 1h.2h significantly reduced (p<0.05), shock 3h significantly increased (p<0.05); FGF2 mRNA expression had increased in the shock 1h,2h shock significantly increased (p<0.05), shock 3h begin to reduce (p<0.05).Conclusion:Shengmaiyin injection of endotoxin shock has a protective role in lung tissue, which may be by way of increased mean arterial pressure, lower mortality, reduce the water content of lung tissue, increased SOD activity, reduced MDA formation, inhibition of iNOS expression generated a large number of NO and increased FGF2 mRNA expression, the expression of NOS1 mRNA increase of the total antioxidant capacity, to endotoxin shock and lung injury, indicating that control Yiqihuoxue detoxification method is an effective shock treatment of early lung injury. |
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