Yam Polysaccharide

Purpose.Rhizoma Dioscoreae is a kind of precious medicinal plant,also is a kind of common food.The seed piece of its medicine use position is rich of starch,polysaccharide(include phlegmatic temperament and glycoprotein),protein chemical composition.Modern medical science research indicated that polysaccharide has many biological activitves,and the main efficiency component in Rhizoma Dioscoreae is polysaccharide of Rhizoma Dioscoreae. Therefore,explicit its chemistry composition and content,determine its making craft and quality control method,can further reveal Rhizoma Dioscoreae's medicinal use value,utilize potential and economy value,provide necessity of basis for Rhizoma Dioscoreae of development.Material and method:In this article,crude drug of Rhizoma Dioscoreae is raw material,adopt the extract method is extract in water and sink in alcohol,to deteremine the best extract craft.Adopt trabeculum chromatographic analysis antigenic depurate the extracted polysaccharide,and study it's monosaccharose composition.In the meantime,with Rhizoma Dioscoreae polysaccharide extract craft for foundation to establish assaymethod.In the end,study the pharmacodynamics of extracted Rhizoma Dioscoreae polysaccharide.Results and Conclusion:1.Through monfactor experiment,I decide the craft parameter of Rhizoma Dioscoreae polysaccharide extract in water and sink in alcohol,check in rthogonal experiment.After combining efficacy,conclude the best Rhizoma Dioscoreae polysaccharide extract craft: dosage liquor ratio 1:20(g/mL),diffusion time for 4 hours,diffuse temperature is 100℃, diffusion times for 3.2.With the best extract craft for foundation,carry on assaying towards being 32 different places Rhizoma Dioscoreae polysaccharide.The result manifestate:the content of different places Rhizoma Dioscoreae polysaccharide is different.Among them,Guizhou is 3.5223%;Dandong in Liaoning is 0.1894%.Content determination with phenol-vitriolic colorimetry,the method is convenient and easy,reproducibility good,the average recovery is 99.6%,RSD=2.00%.(n=5)3.Rhizoma Dioscoreae polysaccharide extracted in water and sink in alcohol,through DEAE-cellulose DE-52 column chromatography gradient separation to get three different parts of A,B,C.Adopt DEAE-Sephadex column chromatography gradient depurate A,to get Rhizoma Dioscoreae polysaccharide A′.Through Sepharose CL-6B gel columnchromatogramphy to verify the purity of A′,phenol-vitriolic colorimetry persuit detect,the result indicated that Rhizoma Dioscoreae polysaccharide A′is a uniform polysaccharide.Through lamella analysis,this polysaccharide A′is compose of glucose and rhamnoseg.4.Through the extraorgan activity of income chemical composition indicate that, Rhizoma Dioscoreae polysaccharide has antioxygen activity,and can raise mouse immunologic function,depress the blood sugar of the diabetes mice.