Forskolin Separation Of Purified

Coleus forskohlii Willd. is the only plants all known that contains forskolin. Forskolin (FSK)is the strongest activator of AC that extracted from botan.It can regulate many kinds of cyto-function in vio in the way of activating AC directly and raise cAMP of many histiocyte.The most important issue in this study was preparing the standard reference material.3 simple substance were isolated from Coleus forskohlii Willd. and were identified as following: Comp I was forskolin; Com II was 2,6,11,15-tetramethyl-2,4,6,8,10,12,14- seven alkene-1,16-di-aldehyde,a red needle crystal ,which can influence the colour of FSK; Com III was 7β-acetoxy -6β, 9β-dihydroxy-1,11,8,13-diepoxy 14-alkene -labd,a colorless needle crystal,which was the main impurity in the production of FSK.Comp II and CompIII were discovered in Coleus forskohlii Willd. and its family firstly. Based on the process of biosynthesis, its has another regularity, it is composed by two monoterpene with its end i.e, while other diterpenes is composed by head and end. It is a new sample in phytochemistry biosynthesis.A self-made monomer of FSK was applied to further the study on the production of FSKwith the methond of silica gel laminar analysis and the recrys of ligarine and acetic ether,to standardize the quality control of Coleus forskohlii Willd's extractive,to improve the mensuration of FSK's contents by HPLC and to settle foundation for the purity decision and technology research of FSK.Based on the quality control of FSK ,a method of preparing extractive of Coleus forskohlii Willd in industry ,rich in FSK,was studied initially.If acetic ether : ligarine were 1: 8-10,and extractive : gel silica were 1: (3-5), the purity of the products could be 60%-80% and that could be 90%.if use acetic ether and ligarine to decolor and recryst. Meanwhile, this study showed that Girard T was nonapplied to isolate and purify FSK,which settle the foundation to further the technology research.