Liver Clear Of Toxic Eliminate The Impact Of Particles On The Acute Liver Injury In Rats Lps And Tnf-¦Á, Il-6 Levels

Purpose: In order to observe the effect of Duxiao Ganqing Keli which fluences liver function consisting of ALT,AST,LPS,IL-6,TNF-a in the animal model of experimental acute liver injury in rats and effect of Duxiao Ganqing Keli to gastrointestinal function,furtherly to discuss the therapeutic effect and mechanism in ALI.Method:1.Dividatur the rats into 6 groups randamly:blank array,model array,Lactitol array,the diminutive,midst,large dose of Duxiao Ganqing Keli.2. the drugs Duxiao Ganqing Keli.were given to the latter three groups and Lactitol .we given to positive control group until 7days.The normal group was not any treatment and the model group through stomach feeding.by physiological saline.3. intragastric injection5days after.Methods To induce the acute liver injury the rats were given intraperitoneal injection with. TAA.except the normal group.4. Animals were killed 48 hours after being grafted, abdominal aorta blood was drawn, we test liver function and Observe the contents of LPS,IL-6,TNF-a in hypothalamus of the acute liver injury rats model with enzyme linked immunosorbent assay (Elisa) We observe the effects of LPS,IL-6,TNF-a by Duxiao Ganqing Keli treatment of acute liver injury on experimental in rats.5.We applied electron microscope to observe the developing changes of liver pathobiology of the HE rats model.6. Observe the effect of carbon particle propelling detection to observe the propellent function of small intestine in mice after Duxiao Ganqing Keli 's treatment.Result:1 The effect on the liver function,LPS of acute liver injury rats model after Duxiao Ganqing Keli 's treatment:All the indexes decreased notably after treatment to the therapeutic groups than the model array's ( P<0.05),and liver function,LPS decreased notably in the midst,large dose of Duxiao Ganqing Keli, there were significant differences than the model array's(P<0.01). It showed certain improvement after Duxiao Ganqing Keli 's treatment. 2 The effect on the IL-6,TNF-a of acute liver injury rats model after Duxiao Ganqing Keli 's treatment:The indexes those were IL-6,TNF-a decreased notably after treatment to the therapeutic groups than the model array's ( P<0.05),and IL-6,TNF-a decreased notably in the midst,large dose of Duxiao Ganqing Keli, there were significant differences than the model array's(P<0.01), the diminutive one decreased too, But it was no difference in the control group.It showed certain improvement after Duxiao Ganqing Keli 's treatment.3 The effect on the liver pathobiology of acute liver injury, rats model after Duxiao Ganqing Keli 's treatment:The hepatocell degeneration and necrosis and delaying the formation of pseudolobe in the positive control group,the diminutive,midst,large dose of Duxiao Ganqing Keli . It indicate that Duxiao Ganqing Keli can reduce the hepatic injury and protect the liver when the liver is injured in acute liver injury rats. 4 The effect on the peristalsis experimental rats model after Duxiao Ganqing Keli 's treatment:The treatment groups are better than the contrast group in the carbon powder's advance percentage(P<0.01,P<0.05). The midst,large dose of Duxiao Ganqing Keli have remarkable significance in contrast with positive group . There is preferable effect of intestinal peristalsis to Duxiao Ganqing KeliConclusion:1 We can produce the acute liver injury model of rats successfully by the rats were given intraperitoneal injection with TAA.2 The consequence to the research manifest that:The consequence to the research manifest that:Duxiao Ganqing Keli can improve hepatic function,LPS,IL-6,TNF-a,liver pathobiology of the acute liver injury rats in a certain extent,the midst,large dose of Duxiao Ganqing Keli is efficient. On the whole, it is effective than the positive control group.3 Duxiao Ganqing Keli is a sort of active drug used to prevention and cure the acute liver injury,and is worthy of further study and development.