| Objective:Prostheses for mandibular defects caused by various factors had been a topic which most scholars were studying on. At present the materials used in the bone damage repair were mainly the synthesized materials, the natural derivation materials and the compound materials. This experiment was to observe the effect of a compound material put Chinese medicine Drynaria into the HA/CH compound material, and to provide the theory basis for the clinical practice.Method: 1. Experimental material preparation: the Drynaria, natural hydroxyl apatite/shell polyose compound materials used In this experiment were provided by Materials Engineering college of Jiamusi University. Allocated proportion method: poured the mixed powder (natural hydroxyl apatite /CH is 8/2) into the shell polyose solution, stirred by the magnetic force for four hours, stirred well-distributed by glass rod to make it disperser evenly, obtained the thick solution. Poured the solution into in the mold, put it in the refrigerator(precooled for 24 hours in - 85℃), took is out, put it in the low temperature freezing box(cryodryed under vacuum state for 48 hours ), then took it out and obtained the compound porous material.2. Animal experimentation: Formed the bone damage model in 48 New Zealand rabbit's mandible. Divided the domestic rabbit into 4 groups according to time of executation after surgery, then took out both side of mandible, divided them into 4 groups, in which experimental group were respectively the davallia(powder-form)/natural HA/CA(Agroup), davallia(dosage-form)/natural HA/CH(B group), control group are modified HA/CH (C group) and not modified natural HA/CH (D group). Executed the domestic rabbit in batch 2, 4, 8, 12 weeks after surgery, took the mandible and observed cicatrization situation, and carried on X piece and the histology slice observation.results: The clinical process was good for the experimental animal after surgery. After implanted the compound materials the tissue slice showed that its inflammation and rejection reaction around the material accorded with the general rules of inflammation and rejection reaction; adverse reaction had not seen by CT.①Experimental group's davallia/natural hydroxyapatite/shell polyose compound materials could basically repair the bone damage 12 weeks after transplanted. The difference had the significant meaning.②The experimental group's davallia/natural hydroxyl apatite/shell polyose compound materials repairing effect after 4, 8 weeks was obviously superior to the control group of natural hydroxyl apatite/shell polyose compound materials. Conclusion: Natural hydroxyl apatite/shell polyose compound materials behaved good biocompatibility, biological security and osteoinduction, which suggested that the compound materials could be used in repairing bone defect. |
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