Corn To Be Anti-gout Screening Of The Active Ingredient

Objective To screen the active principle which can resist the gout of stigmata maydis.Methods: Screening and detecting the active principle which can resist gout of stigmata maydis by using the assay method of xanthine oxidase (XO) in vitro. Through the method of enriching and sublimating by macroporous resin, the active principle of stigmata maydis is extracted and abstracted. Meanwhile, depressant effect of XO is surveyed and evaluated from the extractives of different parts. Compare isolate III with antigut which is took as the positive control in activity.Results: Enriching by macroporous resin, the Isolate III can conspicuously restrain the metabolic process of XO. The IC50 of Isolate III is 6.23mg/L by Method uricogenesis. The IC50 of antigut is 1.10mg/L.Conclusions: By using the assay method of xanthine oxidase;xanthinoxidase in vitro, screening and detecting the active principle which can resist gout of stigmata maydis further, the Isolate III stigmata maydis is found that can restrain the activity of XO and can make a sthonger effection to restain the metabolic process of XO.