Preliminary Functional Studies Of The Of People Abhd6 Gene And The Relationship Between Cancer,

Abhydrolase domain containing (Abhd) gene was a small group belongs toα/βhydrolase superfamily. It is also one of the largest known protein superfamilies. Although their members don't show high sequencial identities between each other, they are classified into one family for their structural similarities. They share the same fold -α/βhydrolase fold. Known members of this group are all found to be involved in important biochemical processes and related to various diseases.In this paper, we focus our research on an unknownα/βhydrolase domain containing (Abhd) , Abhd 6. Abhd6 gene is located on chromosome 3p14.3. The 2364 cDNA sequence (Accession No. NM₀20676) has an ORF which encodes a 337 residue protein, from which 98-322 amino acid is an ABH domain. hAbhd6 and mAbhd6 have high similiarity with each other on both conserved domain, amino acid sequence and advanced structure. Phylogenetic trees of human and mouseα/βhydrolase domain containing genes based on amino acid sequences were similar in topology. In both trees, Abhd6 showed relatively close phylogenetic relationship with Abhd4 and Abhd5, who were lipases involved in regulation of neutral lipid metabolism. We found the widespread tissue distribution of ABDH6 and its differential expression among 7 tumor cell lines. The expression profiles in liver cancer and brain tumor greatly differed from the corresponding results in MTC, where Abhd6 expression was highly expressed. We can not detect similar changes among normal liver cell line and 4 different liver cancer cell lines.In order to unveil Abhd6's function and its relationship with tumorigenesis, we also carried out further experiment including: subcellular location, prokaryotic expression, and flow cytometry to detect the influence of Abhd6 gene on cell cycle. Although the results are not conclusive, they still can give us some clue to proceed.