The Porphyrin Near-infrared Fluorescent Molecular Probe Synthesis And Properties Of,

Near-infrared fluorescence probe was applied well in the medical imaging,In the NIR spectral domain,light scattering in tissue decreases with the reciprocal of the fourth power of wavelength(λ-4),and the NIR spectral domain provides substantial optical window where hemoglobin and water absorption are minimal.With the increase in the wavelength,the rapid decrease about the Raman scattering;compared to the conventional fluorescence(λ＜600nm),biological sample matrix light absorption or fluorescence intensity is very small,thus greatly reducing background interference in the NIR spectral domain(λ＞600nm).Therefore there will be a higher detection sensitivity for the near-infrared fluorescence molecular probe.Up to now,Cyanine,Phthalocyanine,Acid-ching,Thiazide and Oxazine are commonly used as near-infrared fluorescence molecular probe,however their poor biocompatibility and teduious synthetic proecdure limit their wide application.In this paper,we used porphyrin,a natural chromophore which has good biocompatibility,as building block to construct novel NIR fluorescent probe.Two novel NIR porphyrin probe has been achieved.First a butadiyn linked porphyrin, bis{[5,5'-10,20-bis(phenyl)-15-(4-pyridyl)porphyrin]Zinc(Ⅱ)} butadiyne,was constructed through acetylentic coupling reaction,which shows strong electronic absorption in the NIR spectral region(λ＞700nm) and a fluorescent emission at 700nm.Second annulation porphyrin dimer,meso-meso,β-β,β-β,triply linked-[10,20-bismethoxyphenyl) -15-phenyl]Zn(Ⅱ)-Diporphyrin was synthesized by oxidative coupling of its corresponding monomer,and this compound show absorption in the NIR spectral domain(λ＞600nm).These compounds pave the way to the NIR porphyrin fluorescent probe.