Rna Interference Of C-kit K562 Cells Into Nude Mice And The Allylation Dextran Gel Synthesis

Objective To study the effect of short interference RNA(siRNA)on the expression of c-Kit in K562 cells.Methods Plasmid pSilencer-kit expressing c-kit shRNA was constructed. The recombinant plasmid was transfected into K562 cells by lipofectamine. The cellular c-kit expression was detected by RT-PCR. To analyze the tumorigenic ability, we measure the tumor size in nude rats which had been injected with K562 and transfected K562 cells.Results shRNA could significantly reduce the level of c-Kit mRNA. When transfected with pSilencer-kit, the expressions of c-Kit mRNA were inhibited by 81.2 % (P<0.01).Conclusion The vector which express c-Kit shRNA was successfully constructed. The RNA interference can effectively inhibit the expression of target gene c-Kit, and can be potentially useful in gene therapy of c-kit related cancers.