Kidney Deficiency Rat Model Of Renal Aqp2 Expression Change And Its Impact Factors

Objective: This study on expression change of the water channel protein 2 (Aquaporin2, AQP2)in kidney epithelial cells of the kidney-Qi deficiency model rats and its effects on the factors, aims to provide experimental scientific basis of the"the kidney governs water metabolism"theory of TCM.Method: Twenty SD rats are randomly divided into the control group and the model groups ,each is 10 ;Through the method of intraperitioneal injection of amikacin to creat the rat model of kidney-Qi deficiency;CREA and UREA are detected with Enzymatic ;Using the mothod of radioimmunossay to measure plasma ET-1 level; Using the method of HE staining to observe the state of kidney collecting duct; Using the mothod of immunohistochemistry and Western blot to detect the protein expression quantity of aquaporin-2 in collecting duct principal cell; Using the method of PCR to detect the mRNA expression quantity of aquaporin-2 in the kidney.Result: Compared to the control group, the rats of kidney-Qi deficiency had the evident symptom of diuresis(P<0.01) and the lower specific gravity of urine (P<0.01); CREA and UREA of the model group's rat is more than that of the control group (P<0.01);the rat model of kidney-Qi deficiency ET-1 of plasma level increase significantly in the rat model of kidney-Qi deficiency(P<0.05); HE staining of collecting duct under the light microscope displayed: collecting duct shows no change; the mRNA and protein quantity of aquaporin-2 in collecting duct principal cells of kidney deficiency rats is less than that of the control group(P<0.01)Conclusion:1. The expression of protein of aquaporin-2 decrease significantly in renal collecting duct in the the kidney-Qi deficiency model rats.2. The expression of mRNA of aquaporin-2 decrease significantly in renal collecting duct in the the kidney-Qi deficiency model rats.3. The concentration of plasma ET-1 increase significantly in the the kidney-Qi deficiency model rats.