Aconite Compatibility Rhubarb Regulation Of Herbal Substances Basic Research

Objective:Study various factors(complication proportion,decoction time,ahead decoction or later decoction) in RALP and RRR traditional complication how to affect index component content.Attempt to initially uncover the key factors and material basis of herbs properties regulation about RALP&RRR cold-warm complication.Provide scientic explanation about RALP&RRR tradional complication theory which can change cold-purging into warm-purging function.Methods:Apply modern analysis technoledges such as UV,HPLC,TLCS etc.to study the complication of Radix Aconite Lateralis Prepapata(RALP) and Radix et Rhiaoma Rhei(RRR). Taking total alkanoids which is consided the warm property component of RALP and total anthraquinone which is consided the cold property componetnt of RRR as index to establish a polylevel quantitative evaluation system.The system which include total alkanoids-ester alkanoids-diterpenoid alkanoids of RALP and total anthraquinone-free anthraquinone-bind anthraquinone of RRR can help study index component content under different complication proportion and decoction time conditions.The study is carried out at 2 levels:herbs complication and active extracts complications.Determining the content of RALP alkanoids component and RRR anthraquinone component to affirm the active constitutions variation rules at different complication relationship.Results:1.At herbs complication level,the increasing of RRR can promote RALP total alkanoids content;when RALP complication RRR at 1:0.5,it can decrease diterpernoid alkanoid at largest extent.Decoction time increase can elevate total alkanoid and decrease diterpernoid alkanoids. 2.When RALP complication RRR at 1:1 and decrease decoction time can both promote bind anthraquinone content.3.At active extracts complication,when RALP total alkanoids complicates RRR total anthraquinone at 1:1 can increase total alkanoids content.RRR polysaccharide can also increase total alkanoids content.On the contrary,tanni can decrease toal alkanoids content.With the decoction time prolong diterpernoid alkanoids decreases,RRR polysaccharide,tanni both can cause diterpernoid alkanoids decrease.4.The RALP total alkanoids increase can cause total anthraquinone decrease,but it has slight effect on bind anthraquinone.RALP polysaccharide has a similar effect upon anthraquinone.Decoction time has slight effect on total anthraquinone but can heavilily affect bind anthraquinone,when decoction time exceed 30 minutes the bind anthraquinone decrease obviously.Conclusion:RALP warm constitution—alkanoids can duplex regulate RRR cold purging constitution—total anthraquinone and bind anthraquinone.RALP polysaccharide,complication proportion and decoction time is the key factor for RALP regulating RRR cold purging constitution.