The Clinical Significance Of Non-small Cell Lung Cancer, Epidermal Growth Factor Gene Mutations

Objective:To explore the gene mutation of EGFR in non-small cell lung cancer and evaluate the new technique to discover mutations in exon 19and21.Methods:A total of 70 cases was enrolled in this study.The tissue DNA was extracted and the EGFR gene in exon 19 and 21 was subjected for PCR amplification.The mutation of exon 19 and 21 was analysed with clinical data.Results:Mutations were found in 29 cases of 70(41.1%)lung cancer samples,with 20(20/29cases,68.9%)in the exon 19 and 17 (17/29cases,58.6%)in the exon 21.19(65.5%)cases of mutation were detected in adenocacinoma,with 48.7%of detectable rate. 6(20.6%)cases in squamous carcinoma,with 26.9%.4(13.7%)cases in alveolar cell carcinoma,with 80.0%.The EGFR mutations were more frequent in female than male patiants,in adenocacinoma and alveolar cell carcinoma than other hostological types.Conclusion:EGFR mutation is a tumor-specific somatic abnormaity. It is frequently detected with exon 19 and 21,in female,patient without smoking and adenocacinoma.