Preliminary Study Of The Mechanism Of Cm Anti-diabetic Effects

In this paper,the antidiabetic effects of CM,a newly synthesized agent,were investigated to evaluate the antidiabetic effects in CM-treated diabetic animals.Two diabetic models were used:(1)An aUoxan-induced model in which diabetes was produced by Alloxan(50mg/kg,i.v.),then rats were treated with CM(300,100,33 mg/kg)for 14 days;(2)AKK-mouse model in which diabetes was induced by a sustained high lipid diet, then mice were treated with CM for 4 weeks.The hypoglycemic effect of CM exceeded that of Metformin while the hypolipidemic effect was similar.In addition,CM reduced the insulin level of the KK-mice,suggesting that CM improves pancreaticβ-cell function.The effects of CM,Metformin and Cysteine on the antioxidant defense system in the two animal models was also studied.The malondialdehyde(MDA)levels and superoxide dismutase(SOD)levels were determined to provide evidence for oxidative stress,All the groups of animals given CM,Metformin and Cysteine exhibited less severe oxidative stress than the diabetic group.Then,several key antioxidants such as reduced glutathione(GSH),catalase(CAT)and were measured.CM restored the activity of all these agents to nearly normal values.At the end of our study,the animals were sacrificed by decapitation and the liver,kidney and pancreas were weighed to allow investigation of organ edema.The results obtained showed that CM corrected the organ edema of the diabetic rats.We also found CM stimulates AMPKαphosphofilation in HepG2 cells and restores cell viability in high-glucose-damaged NIT-1 cells.All these findings suggested that CM has a protective effect on the antioxidant defense system andβ-cell dysfunction in Alloxan-induced diabetic rats.All these results suggest that CM is a potential candidate for the future treatment of diabetes.