Light Leaf Cacalia Wind Pharmaceutical Research And Three Macrocyclic Polyamine With The Matter Biomimetic Catalytic Performance

Ainsliaea glabra Hemsl. is one genus of Ainsliaea DC, which also can be name as Xin feicao, Shi fengdan. It usually can be found in the edge of forest or under the slope of hill at the altitude of 800～1000m. Ainsliaea glabra Hemsl. Plants distribute in the midstsouthern part of Sichuan and northeast of Yunnan. It has been used as a folk herbal drug in China. It is mainly used for the treatments of the pain of rheumatic, fracture and so on. In addition, it also has effection on antibiosis, anti-inflammatory, antibechic, resolving phlegm, enteritis, dysentery and relieving pains.The chemical researches of Ainsliaea plants discover that the sesquiterpenes intr-aesters compounds in the plants of Ainsliaea show intensive physiologic activity of anti-tumor and oxidation, resist rheumatism, etc. At the same time, pharmacol studys and clinical application indicated that Ainsliaea plants have good anti-inflammatory action. Therefore, there is an extensive market foreground in the space of developing some new medicines to cure cancer, rheumatism and anti-inflammatory from the medicinal plants of Ainsliaea.Currently, there are still less study on Ainsliaea glabra Hemsl. . So, we should keep with the opportunity to study it, and lay stress on the protecting, developing, making use and studying on the resources of the medicinal plants.In this paper we worked on Ainsliaea glabra Hemsl.' s constituent isolation, sturcture determination and quality control on the basis of the antecedent research. With percolation method to extract and isolate the effective composition, we get petroleum ether, chloroform, ethyl acetate and n-butanol. Then we adopt chromatographic methods to isolate chloroform, and get two compounds from it and identificate them as luteolin and 4, 5-hydroxy ilicic acid methyl ester by the ¹H-NMR, ¹³C-NMR, DEPT.The research enrichs the study of Ainsliaea plants and provides scientific reference for developing Ainsliaea plants. Artificialnucleases have been proven to be efficient tools for the footprinting and sequence-specific targeting of nucleic acids. The development of reagents which hydrolytically cleave nucleic acids under mild conditions is currently attracting great interest in the field of artificial nucleases. While many reagents have been successfully applied to RNA hydrolytic cleavage, there have been fewer successes with DNA because of its relatively high hydrolytic stability. Metal ions play important roles in many enzyme and all ribozymemediated examples of these scission reactions. Small metal complexes that promote the hydrolytic cleavage of DNA, therefore could be useful not only in molecular biology and drug design but also in elucidating the precise role of metal ions in enzyme catalysis.In this dissertation, three typical macrocyclic polyamine metal complexes have been investigated. They're functional rown ethers macrocyclic polyamine (mononuclear), Chiral dinuclear macrocyclic polyamine complexes (binuclear) and multinuclear macrocyclic polyamine (multinuclear).The hydrolysis cleavage of plasmid DNA (pUC19) by all three macrocyclic polyamine metal complexes has been investigated, and the results revealed that these complexes could act as powerful catalysts for the cleavage of plasmid DNA under physiological conditions. Activity of cleavage mainly based on structure of compound, pH, density and so on. Monocaryon is the best, and then the dicaryon, the polynucleation last. We also found that all thress copper complex are better than their zinc and cobalt complex. The cleavage Activity of functional rown ethers macrocyclic polyamine, and multinuclear macrocyclic polyamine can be inhibited by oxyradical inhibitor, so, we can conclude that their cleavage is thru oxyradical way. In other hand, chiral dinuclear macrocyclic polyamine complexes can not be inhibit by oxyradical inhibitor, it may be implement in another way.