Centipede Notoginseng Chemical Composition Of The Structure-activity Relationship Study

At present, the research and development of bioactive of Chinese traditional and herbal drugs is domestically a hotspot.With an in-depth study, a great deal of monomers are separated. Though some of them are expatiated ,most are not illuminated. So many of monomers are leave unused and unprofitable.So this results in great waste. My paper focus on the four monomers of Wugong sanqi and to find the difference from them. The study adopt a technique.of Cell Culture and use cell to make model.Wugong Sanqi is the dry rhizome of Anemone flaccida Fr.Schmidt. It is distributed in the south part of China ,YiChang region in Hubei also have to distribute and used as folk medicine for detoxication, the treatment rheumatism painful and painful, fall to beat the harm,to expell wind-evil and remove wetness-evil. The main bioactive fraction is proved to be triterpenoid saponins by pharmacotical study and as material of new drug by clinic on Wugong Sanqi. Studies on extraction and isolation of triterpenoid saponins of the main bioactive fraction of Anemone flaccida Fr.Schmid. Five triterpenoid saponins were isolated from the validity part, named as Anemone flacccidoside W_0a,W_0b,W₁,W₃,W₂.Because there are short of W₂.My study have to focus on W_0a,W_0b,W₁,W₃.Rheumatism arthritis(RA) is one of the most familiar rheumatism.It is distributing all over the world and has a high morbidity and disability rate.The pathogeny and mechanism of RA is very compex.Abnormality of cell-mediated immunity is thought of as the key of RA.T cell is the most one between inflammatory cells in synovial joint.The immune damage which is brought by excessive activated T cell aggravates inflammatory reaction. When antigen trespass on the body ,the lymphocyte is activated and multiplicated.The T-cell response can resist the antigen,but when T-cell produce multiply by tissue, parts, cells, The state of exceeding what is normal or sufficient,thus it will lead to RA. The activation of lymphocytes by ConA is the same as of antigen. By means of MTT, we found W₁,W₃ can restrain amount of T-cell when abnormity ,but not affect T-cell when well-balanced.RA is a complex autoimmune disorder, characterized by a chronic T-cell response that has evaded nomal control mechanisms.Therefore,the gene involved in the regulation of T-cell response may be primary determinants of susceptibility to RA.CTLA-4 and sFas are key negative regulators of T-cell activation and are considered candidate genes for autoimmune diseases including RA.It has been reported that CTLA-4 and sFas polymorphisms are associated with several T-cell mediated autoimmune diseases. By means of RT-PCR we found W₁,W₃ can affect the expression of CTLA-4 and sFas.So they can possibly fight back RA.