Pueraria Flavonoids Extraction And Separation Of Its Quality

Radix Puerariae, a Cucumis sativus herb plant of Leguminosae, For traditional Chinese medicine in common use, has a solution muscle abatement of fever, promotes the secretion of body fluid to quench thirst, Sun Micro antidiarrheal is used to cure illnesses such as myocardial infarction, arrhythmia cordis, hypertension wait for effect, clinical mainly. Research indicates the modern pharmacology, whose active constituent is flavonid compound. Fingerprint collection of illustrative plates and contents analysis picking up a thing's have carried out a thesis on main Puerariae flavonid component, always flavonid handicraft extracting a purification, flavonid studying.Has adopt menstruum to follow sum chromatography having been in progress to Puerariae flavonid extracting separation studying, has got 4 monomer components out of, they are daidein, daidzin, puerarin, 3′-melhoxypuerarin. This several kinds compound are that the root of kudzu vine has the typical active component in always yellow ketone.The purification handicraft has carried out the method adopt the orthogonality experiment on Puerariae flavonid drawing giving selections preferential treatment studying, has ascertained always Puerariae flavonid alcohol back-flow extracting the big sum hole resin purification technological parameter, has tested and a certificate in having been in progress, the stability extracting always yellow thing middle ketone contents amounting to 50% all above, handicraft is feasible. May transit to put to use in production childbirth.The contents having adopt HPLC analysis method, having beenin progress to main Puerariae medicinal material middle active constituent analyse the height having studied, building sensitiveness, reproduce nature regards and strong contents of reliability determining method.Has adopt high-effect capillary electrophoresis to follow the research picking up a thing to Puerariae flavonid having carried out fingerprint collection of illustrative plates′s, has calibrated common 14 peaks, the various technology parameter accords with country medicines and chemical reagents food without exception superintending the relevance request that the administration bureau promulgates, method reproduces nature and the stability fine.