Primary Liver Cancer. Xiaoliu Treated Clinical And Experimental Research

We investigated Chinese medicinal formulae Xiaoliutang's effect oncuring Primary Hepatic Carcinoma through three ways: clinicalobservation and depressant effect in vivo and vitro empirical study. Thesize of carcinoma and biochemical indicator of patients were detectedbesides of the symptoms in the clinical observation. And in the animalexperiment we observed the depressant effect of Xiaoliutang on portabilitytumorous in mouse. We used drug serum to restrain Bel-7402 cells vitrocultured. We try to confirm the depressant effect of Chinese medicinalformulae Xiaoliutang on primary hepatic carcinoma and find it'smechanism.We selected out-patient clinic and admission department patients ofdepartment of traditional Chinese medicine of Union Hospital affiliated toHuazhong University of Science and Technology who suffered primaryhepatic carcinoma. We sieved patients according to "Standard of clinicaldiagnosis and stage of primary hepatic carcinoma" settled in the eighthnationwide hepatoma academic conference in 2001. Fifty five patientswere divided into treatment group and control group randomly. There werethirty patients in treatment group. They took 400 ml Xiaoliutang apozemper day. Twenty five patients in control group use Fluorouracil 500 mg per day by intervenous drop infusion. The observation indicatrix includedamelioration of symptoms, AFP, AST, ALT,γ-GT, Blood-Rt, stabilizationof tumour and adverse reaction. After three months of treatment the resultindicated the symptoms of the patients in the treatment group wereimproved noticeable(P＜0.01). The stabilization rate of AFP of treatmentgroup was 33.33％and the stabilization rate of AFP of the control groupwas 30.43％. There wasn't significant difference between these twogroups(P＞0.05). The effective rate of ASt in treatment group was 35.71％,the effective rate of ALT in treatment group was 35.71％, the effective rateofγ-GT in treatment group was 20.69％. The effective rate ofAST, ALT,γ-GT in the control group was 34.78％, 40.91％, 20.83％. Therewasn't significant difference of the effective rate of AST, ALT andγ-GTbetween these two groups. The stabilization rate of tumour of the patientsin the treatment group was 13.3％. The stabilization rate of turnout of thepatients in the control group was 16.0％. There wasn't significantdifference between these two groups. There wasn't adverse reaction in thetreatment group and the symptom of the patients was improvedconspicuously. It showed that Xiaoliutang could inhibit canceration of livercells. It could attenuate the synthesis of AFP of cancerated liver cells andinhibit growth of the tumour. Xiaoliutang could lessen injury of liver cellsand depress the activity of AST and ALT. It could improve the living quality of the patients and prolong their life.We used bearing cancer mouse (S180 ascites tumor) in the animalexperiment. The mice were divided into five groups randomly. There werehigh dose Xiaoliutang group, middle dose Xiaoliutang group, low doseXiaoliutang group, Fluorouracil group and Sodium Chloride group. Thecourse of treatment was 14 days. The tumor was undermined from themouse and was weighed. It come out that middle dose Xiaoliutang andFluorouracil have obvious anti cancer effect compared with the SodiumChloride(P＜0.05, P＜0.01). The high dose and low dose Xiaoliutanghaven't obvious anti cancer effect compared with the Sodium Chloride(P＞0.05). It suggested that anti cancer effect of Xiaoliutang hadrelationship with its dose.In the vitro empirical study we made the SD rat take Xiaoliutang orally.Then draw its serum. We used the rat's serum contained Xiaoliutang asdrug to interfere the growth of Bel-7402 cells. The cells were divided intohigh, middle and low dose Xiaoliutang groups, Fluorouracil group andSodium Chloride group. The administration time was 96 hours. Weobserved the growth inhibiting rate and morphous of the cells. We detectedthe multiplication of cells by MTT. It came out that 12.5％serum of high,middle, low dose and Fluorouracil could inhibit cell multiplicationobviously compared with Sodium Chloride(P＜0.01). And low dose serum have more inhibitory effect than Fluorouracil (P＜0.05). Serum of 25％ofhigh, middle dose and Fluorouracil had obvious inhibitory effect comparedwith the Solid Chloride (P＜0.01). Low dose could inhibit the cell growthobviously than Solid Chloride(P＜0.05).The inhibitory effect of serum andFluorouracil had no obviously difference(P＞0.05). After 50％serum wasgiven it showed high dose and Fluorouracil had noticeable inhibitory effectthan Solid Chloride (P＜0.01). Low dose serum also had obvious inhibitoryeffect compared with Solid Chloride (P＜0.05). Middle dose serum had noinhibitory effect. There was no distinguish difference among high, lowdose and Fluorouracil (P＞0.05). We could see in the light microscope thatthe cells interfered with serum were aspheric. There were karyopyknosis.Caryotheca and membrane' structure of the cells was damaged. The cellnucleus was split. And there were apoptotic body in the cells. Parts of thecells interfered with Fluorouracil also had apoptosis. The cells of controlgroup were normal. The disposition of caryoplasm was even. There wasn'tapoptosis. It is certified that Xiaoliutang could induce tumor cell apoptosisand inhibit tumor cell multiplication to depress tumor growth. Its effect hadrelationship with its dose and concentration.