Power For Parkinson's Disease Rat Model Of Neurotrophic Factor And Its Receptor Expression

Objective: Parkinson's disease (PD), which is a parapymmid progressive neurodegenerative diseases in centre nerve system, always invades middle-ages and older person and causes their disables. PD's mainly pathology change maintains the death of most of the dopamine neurons which project from tight zone of substantial nigra of midbrain to striatum. PD's mainly symptoms include stilly shacking, bradykinesia, rigidity muscles and abnormal posture and pace. Nowadays, western medicines could not prevent the progress of PD well, but bring about a lot of side effects at the meantime. Operation and gene therapy could not treat the PD well, either. At present, acupuncture as a peculiar, safety, and convenient way has been applied in the treatment of PD and has good effection. The text will study the therapeutical effect and mechanism of the electroacupuncture(EA) on Parkinson's disease (PD), which will offer experimental basis for applying EA in clinic praction of the acupuncture -moxibustion.Methods: Wistar rats were allocated into four groups by random: PD group, EA group, sham-operation group and normal control group. PD model was established by microinjecting 6-hydroxy-dopamine into mesencephalic substantia nigra in the right brain. The same amount of normal saline was injected into the rats of control group. After 4 weeks, Apomorphine was injected into the rats abdominal cavity to induce rotation. The test were repeated every 7 days. Rats who had constant left rotation with a speed faster than 7r/min were selected as successful PD models. 41 successful PD model rats were selected for treatment and randomly divided into two groups, model group and electroacupuncture group. Acupoints of Fengfu and Taichong in EA group were punctured once a day for 28 days. The results were analysed by F-test and q-test.1. Observation on behaviour: 0.5 mg/kg of apomorphine was injected into abdominal cavity to induce left rotation. The number of circles rotated within 30 minutes after injection of apomorphine were recorded, once a week for 4 weeks 2. The contents of glutathione peroxidase, superoxide dismutase and glutathione in the right of substantia nigra: The rat was decapitated and the brain was taken out, peeled off bilateral striatum on an ice cold plate, grinded with precooling physiological saline, centrifugated at 3000r/minute for 45minute, the supernatant fluid was used for detection with SOD, PSH-PX, PSH.3. The positive brain-derived neurotrophic factor(BDNF) neurons content in the right of substantia nigra: the above slice were taken, immune constitution chemical method was adopted to measure the positive BDNF neurons, positive neurons: whose cell plasm should be dyed into tan.4. The positive tyrosine kinase-B content in the right of substantia nigra: the above slice were taken, immune constitution chemical method was adopted to measure the positive BDNF neurons, positive neurons: whose cell plasm should be dyed into tan.5. Statistical analysis: All the data were processed with SPSS11.0 software and expressed as Mean±SD. Q Test was used for comparison beetwin different groups.Result:1. In EA group, the number of rotations after the treatment was lower than before the treatment(P＜0.05), but that didn't change in PD group(P＞0.05).2. The contents of glutathione peroxidase,superoxide dismutase and glutathione on the right substantia nigra in the PD group was lower than the normal group (P＜0.05,P＜0.01, P＜0.01), and those in the EA group was higher than the PD group(P＜0.01,P＜0.01,P＜0.05).3. The contents of brain-derived neurotrophic factor on the right substantia nigra in the PD group was lower than the normal group(P＜0.01), and that in the EA group was higher than the PD group (P＜0.05).4. The contents of tyrosine kinase-B on the right substantia nigra in the PD group was lower than the normal group, and that in the EA group was higher than the PD group (P＜0.05).Conclusion: BDNF plays an important role in protecting DA neurons and maintaining their normal function, and demonstrates the attractive prospect in the PD treatment. EA can obviously prevent the PD mouse to revolve. Its possible mechanism:①EA can enhance the contents of glutathione peroxidase, superoxide dismutase and glutathione, which prevents the denaturation of DA neurons.②EA can enhance the contents of BDNF and TrkB, which can promote the regeneration of DA nurons.