Ranae Antitussive Active Ingredient Chemical Synthesis And Biological Activity Study

1—Methyl hydantoin is a kind of hydatoin compound and also the principal effective component of Rana temporaria chensinensis David.lts structure has been identified. The literature reports indicated that 1—Methyl hydantoin has many kinds of physiologic activities, each kind of different substitution Hydantoin and its the derivative have the widespread pharmacology activeness and application.Recently,the methods of 1—Methyl hydantoin preparation is to extract and separate from Oviductus Ranae.While the method is rather complex, long time and low recovery yield,and waste large amounts of organic solvent.So it's not economic to extract 1—Methyl hydantoin. After analyzing the structure of 1—Methyl hydantoin,it can also be prepared by means of chemical synthesis.This article design the synthesis routine by the method of anti-synthesis and product receiving rate is higher, the purityreaches above 97%.The structures of the targetmolecules determined by melting point,thin layer chromatograph(TLC),IR,~1H-NMR and(13)C-NMR are the same as the standard sample.This synthetic technology has somedvantages:easily available raw material, low cost, easy operation and mild reactionondition. After the reliable product, uses X - ray diffraction research, had determined 1 -ethyl Hydantoin's molecular structure and themolecule space arrangement, through tothe compound atom hybridizationway analysis, explained the 1 - methyl Hydantoinmber the key lengthand the bond angle and so on the corresponding data.Through the experiment study the Oviductus Ranae component 1- methyl Hydantoin's pharmacological action. Uses the experimentthe mouse cough direct by ammonia water, the mouse phenol red experiment, and the research to the normal mouse anti-endurance test. Discovered the Oviductus Ranae and 1- methyl Hydantoin have the remarkable effect to the mouse ammonia water initiation cough,the anti- endurance test confirmed the Oviductus Ranae and 1- methyl Hydantoin can obviously lengthen the mouse swimming and the climb stick time.