Gavage Gynostemma Extract The Content Of The Natural Aging Mice Skin Tissue, H <sub> 2 </ Sub> The O <sub> 2 </ Sub>, Gsh-px Activity In Experimental Research

Objective: The experiment studied giving traditional chinesemedicine GP to the animal to determine whose content of hydrogenperoxide (H₂O₂)of the skin organization, the change of Glutathioneperoxidase (GSH-PX) and the skin ultra structure, to investigate GPeffect on the respect of small rat skin aging, delay a senilitythereby, and lead to skin aging free radical loss theory in clinicalsignificance and modern medicine old and feeble furter knowing andexpounding and proving doctor of traditional chinese medicine to addsupplemental false strengthening the body resistance resisted.Methods: 10 Healthy KM female rats age in 3 months contrasting a groupas youth (A);40 old in natural of KM female rats age in 12 monthswere divided into 4 groups randomly and gavagad respectively, thatis old age oral blank group (B),old age oral gender group (C),oldage irrigates oral GP group (D),old age oral GP and external group(E).A group and B group oral saline (0.8ml/t),C group oral VitaminE (0.2ml/t),D group and E group oral the GP (0.8ml/t),and E groupexternal the GP (0.5g/t),daub area for (3×3cm²), whose thicknessis 8%.Two times per day, altogether 30 day. Adopt pathologicalmechanism to organize the section film production technology, HEdyeing, the structure observing a dermis changes, and analysismethod determines with biochemistry H₂O₂ contents and GSH-PX acti-vity with 100 glossy mirrors.Results: Comparing with youth collation group, H₂O₂ contents arenotable in other four old age rats of groups skin organizationincreasing (respectively P＜0.01, P＜0.05, P＜0.01, P＜0.05), thatGSH-PX activity reduces (respectively P＜0.01, P＜0.01, P＜0.01, P＜0.01);Pathological mechanism section of organization displaysthe state aging naturally; Comparing with oral blank group, old ageoral gender group, old age irrigates oral GP group and old age oralGP external group is equal but reducing H₂O₂ contents (respectivelyP＜0.01, P＜0.01, P＜0.01, P＜0.01), improving GSH-PX activity(respectively P＜0.01, P＜0.05, P＜0.01);And irrigate that the oldage irrigates oral GP group D being compared with each other, usesbesides fitting at the same time to reduce contents H₂O₂ (P＜0.05)for medicine way E group and improves stronger active GSH-PX effect(P＜0.05) exclusively.Conclusion: The GP the oxidation resistance picking up liquid butraising airframe by eliminating a free radical, effects small rat skin organization and feeble oxide loss,reducing the H₂O₂ contentsand the activity improving GSH-PX, delaying the senility that skinorganizes thereby. Oral the GP and external can get up more well todelaying damage, whose mechanism at ease old and feeble to smallrat skin possibility for medicine way is that way can raise bodyinner medicine since inside and outside is the medicine with begiven to concurrently thickness more beneficial to airframe sorp-tion make use of.