| Objective: Through observation of the effects on the neuroendocrine functions of climacteric rat model with interior heat syndrome due to deficiency of yin by ear-apex bloodletting method to investigate its therapeutic effects and mechanism on hot flush and support for the clinical practice.Method: 60 female Wistar rats (180～250g) were randomly divided into 5 groups of normal group, sham-operated group, model group, Diethylstibestrol (DES) group and ear-apex bloodletting(EAB) group. Rats in model group, DES group and EAB group were removed with the ovaries while the sham-operated group with the same operations but without removal of the ovaries. On the tenth day of post-operation, rats in the model group, DES group and EAB group were manipulated with lavage of 50% Fuzi Ganjiang Decoction (Prepared Common Monkshood Daughter Root & Dried Ginger) to prepare interior heat syndrome while the normal group and sham-operated group with lavage of distilled water as contrast. Climacteric rat model with interior heat syndrome due to yin deficiency by method of removal of ovaries and lavage of 50% Fuzi Ganjiang Decoction, were treated with different methods of ear-apex bloodletting, lavage of DES while the control groups with lavage of distilled water. Rat rectal temperature was measured by digital themometor; Rat desquamated Vagina Epithelial Cell was observed under light microscope; Rat uterus was precisely measured after removal and then for calculation of uterus weight index; Rat serum Estradiol (E2), Follicle-stimulating Hormone (FSH) and Luteinizing Hormone (LH) were measured by radio-immunity method; Rat hypothalamus NE, 5-HT and 5-HIAA were measured by High performance Liquid Chromatography with Electrochemical Detection (HPLC-ECD).Result: 1. Rat Rectal Temperature: Rectal temperature in model group had an increasing tendency and obviously increased higher than those in normal group, sham-operated group and DES group (p<0.05). Rat rectal temperature in DES group obviously decreased lower than those in model group (p<0.05). There were no obvious difference between ear-apex bloodletting group and model group. 2. Rat Vagina Epithelial Cell: Vaginal smear results showed a lot of epithelial cells and cuticular epithelial cells in normal group, sham-operated group and DES group while in model group and ear-apex bloodletting group with a lot of leucocytes and polycytes. The extent of cuticularization of the vaginal epithelial cells was obviously lower than that in normal group and sham-operated group while the DES group with higher extent. There were no difference between the EAB group and model group. 3. Rat Uterus Weight Index: Uterus weight index in model group descended extremely remarkable in contrast with normal group and sham-operated group (p<0.001). Index in DES group ascended extremely remarkable in contrast with that of model group (p<0.001). Index in EAB group had an increasing tendency in comparison with that of model group. 4. Rat Serum Hormones: E2 in model group descended obviously than those of normal group and sham-operated group (p<0.01), FSH ascended obviously (p<0.01) and LH had an increasing tendency. In contrast with model group, E2 in DES group and EAB group had an increasing tendency, FSH descended obviously (p<0.05) & LH had a decreasing tendency. 5. Rat Hypothalamus Neurotransmitters: In contrast with normal group, NE in model group obviously increased (p<0.05), ratio of 5-HT/ NE obviously descended (p<0.05) and 5-HT and 5-HIAA had a descending tendency. In contrast with model group, NE and ratio of 5-HT/NE in DES group obviously descended (p<0.05), 5-HT descended significantly (p<0.01) and 5-HIAA/5-HT ascended obviously (p<0.05). In comparison with model group, NE and ratio of 5-HT/NE in EAB group obviously descended (p<0.05), 5-HT descended significantly (p<0.01), 5-HIAA increased obviously (p<0.05) and 5-HIAA/5-HT ascended significantly (p<0.01).Conclusion: Ear-apex bloodletting has functions of lowering rat rectal temperature, LH, FSH, NE, 5-HT & ratio of 5-HT/NE and improving E2 and ratio of 5-HIAA/5-HT. Ear-apex bloodletting method can effect on the neuroendocrine functions of climacteric rat model and has certain effect on treating climacteric syndrome. |
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