Comparative Proteomics Screening For The Identification Of Pancreatic Cancer Multidrug Resistance-associated Protein Of Experimental Research

Objectives:Pancreatic cancer is a lethal disease of digestive system. Unfortunately, most people are discovered too late to surgery, so there are only 15-20% patients of with pancreatic cancer have the opportunity to be operated. Chemotherapy is an important adjuvant treatment to improve the quality of life and prolong the lifetime. However only a limited proportion of patients of pancreatic cancer responds favorably to commonly used chemotherapy reagents. Previous studies have revealed several genetic factors that influence the chemosensitivity of pancreatic cancer such as the gene associated with transport of cell membrane, energy metabolize and DNA repair. Considering the effect of single factor is insufficient because chemosensitivity involves multiple interacting factors that contribute to the overall response. Preciously, our laboratory has screen out numerous genes associated with chemoresistance of pancreatic cancer and hint that the genes maybe fuction as a network. In this study, we employed the technology of proteomics to look for the differential proteins between the two chemoresistant cell lines and parental cell line to investigate the mechanize of chemoresistance and provide a theory for the therapy in the future. Methods:1. The chemosensitivity of pancreatic cancer cell lines was determined by CCK-8 assay.2. Separated the different proteins of each cell lines by two-dimension gel electrophoresis.3. Analyze the reproducibility of gels of SW1990, SW1990/ADM and SW1990/Fu by ImageMaster^TM 2D platinum.4. Compared the spots in the chemoresistant cell lines with which in the parental cell line by ImageMaster? 2D platinum.5. Identified the differential proteins by mass spectrometry and look into the...