Banlangen Polysaccharide Chemistry And Solution Viscosity And Immunomodulatory Activity

Radix Isatidis is commonly used Chinese herbal medicine, it has extensive pharmacological actions, including antivirus,anticancer,strengthen immunity dint etc. Radix Isatidis often with drink a slice and take orally liquid and blunt etc. to used for clinical .The plant of Radix Isatidis in our country is standardized and scaled. Generally the percent of Radix Isatidis Polysaccharide is 24.87%.But Radix Isatidis Polysaccharide was deserted as waste in preparation of Radix Isatidis,and the research of Radix Isatidis Polysaccharide about it's active was not systemic and in depth. ParticularlyBased on physical chemistry and natural pharmaceutical chemistry,theroy and technology in natural pharmaceutical chemistry was used in the research of Radix Isatidis about extraction,isolation,determination of molecular weight, estimation of viscosity of different Radix Isatidis Polysaccharide and , with expect to establish a reliable technique for determining the molecular weight,reveal physicochemical property of Radix Isatidis Polysaccharide such as molecular weight and viscocity. provide theories basis and material foundation for extensive pharmacology, creating new drug or food with Radix Isatidis Polysaccharide and making full use of Radix Isatidis.Phenol-vitriolic colorimetry has been chosen to be the method of mensuration of Radix Isatidis Polysaccharide.It shows that the linear rang was 0.02mg/ml～0.10 mg/ml(r=0.9985).It's RSD% was0.0995%(n=5).the method was convenient,rapid,accurate and suitable assay of polysaccharide.Setting up the scheme of aqueous extract→ultra– filtration→precipitation in ethanol to isolate Radix Isatidis Polysaccharide, the method ultra– filtration can remove small molecule with effective.This method was convenient and simplicity, it was suitable to purificating a great deal polysaccharide.