Hydroxycamptothecin With Methotrexate Inhibition Of Hela Cells, A Comparative Study

Objective To investigate the effect of 10-Hydrocamptothecin (HCPT) on cell proliferation and cell cycle of the cultured Henrietta Lacks strain of cancer cells (Hela cells). Furthermore, throμgh contrast with methotrexate (MTX), try to find out the potential value of treatment for rheumatoid arthritis (RA).Methods Using the MTT method to measure the suck-light degree value of the cells treated with different doses of HCPT at different time and the same doses of HCPT and MTX at the same time, and to count the inhibition rate (IR%); using the flow cytometry with PI staining to detect the changes of cell cycle of the Hela cells treated with different doses of HCPT and the same doses of HCPT and MTX.Results Both HCPT and MTX can inhibit the proliferation of the cultured Hela cells and the effect is dose- and time-dependent. It makes IR% increasing, which has statistic significance (p<0.001). The differences between HCPT and MTX are that the value of IC50 of MTX is smaller than that of HCPT and the maximum inhibition rate of HCPT is about two times larger than that of MTX; Both HCPT and MTX can change the cell cycle and the effect is enhanced with the increasing of the drμg doses(p<0.005). They both act in S phase. MTX can make the number of cells in S phase decreasing and the number of cells in G0/G1 phase increasing, but HCPT can make the number of cells in S phase increasing and the number of cells in G0/G1 phase decreasing.Conclusion Both HCPT and MTX can inhibit the cell proliferation of Hela cells and the effect is dose- and time-dependent. But the potency of MTX is higher than HCPT, and the maximum efficacy of HCPT is larger than MTX; HCPT and MTX can change the cell cycle and both act in S phase, but HCPT act in the middle and late segment of S phase , and it can increase the number of cells in S phase and reduce the number of cells in G0/G1 phase, which sμggests HCPT have potential value in the treatment of RA.