| Cancer is one of the main diseases that threaten peoples' health and life at present. A great deal of clinical practice indicated that the development of cancer could be controlled through early diagnosis and valid treatment to improve the chance of survival. Raman spectrum is an effective method for analyzing substance structure .It can represent the differences of chemical composition and molecular constitution in the tumor cell at molecular level, and these differences may provide some important information for the diagnose of cancer and the analysis of canceration mechanism. Since the activation of telomerase has been found universally in human cancers and telomerase is the key for the existence of tumor cells and is also the necessary condition for the proliferation of tumor tissue, telomerase has been explored as a promising biomarker and target in cancer gene therapy. It is at the exploring stage to the research for regulatory mechanism of the telomerase activity, and the biochemical characteristic and antitumor mechanism of the telomerase inhibitors should be further researched.In this thesis, unicell tweezers Raman spectroscopy is used to study the hepatocyte LO2,hepatoma carcinoma cell SMMC-7721 and nasopharyngeal epithelial cell HBE,nasopharyngeal carcinoma cell CNE2. Characteristic spectrum can be founded through the comparing and analyzing of the spectrum. To discuss the mechanism of telomerase inhibitors on cancer, hepatoma carcinoma cell SMMC-7721 and nasopharyngeal carcinoma cell CNE2 treated by 8 telomerase inhibitors were studied. The molecule changes can be seen from the treated cancer cell. The main results are as follows:1. Experimental results have demonstrated that Raman spectra of malignant cells were significantly different from those of normal cells. Biological molecules such as DNA, protein, lipid undergo a series of changes in content or structure .In hepatoma carcinoma cell SMMC-7721 and nasopharyngeal... |
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