Effective Parts Of The Honey Locust Cancer Research

(The study on the anticancer and effective part of Chinese honey locust)ONE The Aim of the Study:The antitumour function of Chinese honey locust(one kind of plant medicine) was found in the study of antitumourfiltration,and it was approved that the adhesive plaster of Chinese honeylocust has definite anticancer effect by the antitumour experimentationand clinic study.Basing on it,the aim of the study is adopting modernseparation methods to separate adhesive plaster and study antitumourfiltration in order to confirm the anticancer part of Chinese honey locust.Studying definite effective part,including distill and purificationtechnics,quality standards, stability,antitumour pharmacody andtoxicology,in order to synthesis evaluate the anticancer effect andtoxicity of the effective part.TWO Methods and Results of StudyFirst Adopting different methods to separate adhesive plaster intosaponin ingredient and other parts.Second It was found that saponin part had remarkable repress tumourfunction by resisting animal S180 filtration,so saponin part was effectivepart.Third In the light of saponin,it is approved that the distilltechnics of saponin of Chinese honey locust was the best by positivejoint and excellent choose experiments of three ingredients and threelevel.Fourth Adopting macroporous resin to separate and purify diatilledcoarse saponin part,in order to make effective part have 60% saponin.Itaccords the technology demand that effective part must overtop 50% todeclare this kind of new drug.Rarefied saponin part is called "saponinextract".Fifth According to technology demand of declaring new drug,studyingquality criterion of saponin extract to map out the quality criterion anddrafting narration of saponin extract.Sixth According to the stability demand of declaring new drug, doingsome stability experiments to study the stability of saponin extract.Asa result,the saponin extract is stable after deposited for 18 months inthe room temperature.Seventh The saponin extract has no remarkable toxicity respond bymaking some experiments of acute toxicity.It was measured that LD50 was3.97±0.45g/kg,and proved that the studyed clinic dose was safe.Eighth antitumour pharmacology experiments:(A) The experiments of restraining HL-60 and SGC7901:The experimentsof respressing tumour outside body indicated that saponin extract hadremarkable restraining function and domeno effect relation of time anddose vs two kind of cells.The LC50 was 30.36μg/ml and 2.29μg/mlseparately vs HL-60 in 24 hours and 72 hours. The LC50 was 190.98μg/mland 6.29μg/ml separately vs SGC7901 in 24 hours and 72 hours.(B) The experiments of antitumour mostly pharmacology:Using themedicine to cure mouse′s S180,liver cancer and study the affection toimmunity function of body.As a result,the extract could restrain S180well,and the rate of antitumour was 52.5%～67.8%(P<0.01).It can restrainmouse ′ s liver cancer(P<0.01),prolong the living time of miceremarkablly,enhance nonspecificity immunity function of functional miceand grateful hump mice(P<0.01),and remarkablly increase the effect ofcyclophosphamide of low dose(P<0.01).THREE Innovation and Significance of Research FruitFirst The anticancer function of Chinese honey locust is foundfirst all over the world,and it belongs to innovate new drug study.Thedifinition of active part of saponin can be declared as creative newdrug.That the separation of effective part and the difinition of activeingredient can be developed into anticancer new drug of ChineseTraditional Medicine First.Second It is first all over the world that people study antitumourpharmic, pharmacology and toxicology of Chinese honey locust.It confirmedanticancer function of the medicine,and provided perfect technology datafor anticancer study of the medicine.Third The rate of antitumour of Chinese honey locust's saponin partvs S180 is 67.8%,and overtop the rate of antitumour of cyclophosphamidewhich is 50.36%(P<0.05).It is very rare in the anticancer ChineseTraditional Medicine and natural plant medicine at present,and break thetraditional conception that the anticancer curative effect of ChineseTraditional Medicine is not as good as Western medicine.Fourth At the present time,the effective ingredients of anticancerChinese Traditional Medicine and natural medicine are mostly alkaloid,naphtha, amylose and so on.There is few report about the anticancer studyof saponin ingredient.The confirm that the saponin ingredient is theeffective part of anticancer provides new thought for the research ofanticancer medicine.Fifth Our province is one of main producing area of Chinese honeylocust.The resourse is richful,and can be gained easily with low price.Thecontent of effective ingredients of Chinese honey locust is high.It isan obvious advantage vs most Chinese Traditional Medicines that they havea few effective ingredients and they are expensive.FOUR ConclusionThe saponin part of Chinese honey locust has obvious restrain functionvs many animal tumour,and can remarkablly increase the effect ofcyclophosphamide of low dose, and has no obvious toxicity.So the medicineis prospective creative new drug which has good effect with few toxicityand independent knowledge property right.