Molecular Epidemiological Studies Of Epidemic Hemorrhagic Fever Virus For Rodents In Henan Province

Objective: Using the methods of the molecular epidemiology, Hantaan viral nucleic acids were typed by Reverse transcription-polymerase chain reaction(RT-PCR) and the sequences of amplified partial genetic fragnments was used toanalysis. To investigate hantanvirus infection of captured rodents in different disdistrict of Henan and to type hantavirus using molecular technique. To study genotypic distribution and diversity and new type. To investigate genetic phylogenetic development and evolution. To offer essential scientific evidence for prevention and cure hemorrhagic fever with renal syndrome.Methods: The captured mice were classfied and the density of distribution was calculated. using direct immunofluorescences detected the tissue slice of the rodent lungs. Partial M and S segments were amplified with nested RT-PCR using hantavirus genotype-specific primers, and then were sequenced , analyzed and compared with other known sequences. To draw phylogenetic tress by Phylip sofeware and study evolutional relation. Genetic subtypes and geographical distributions were ascertained.Results: Atotul of 695 animals were captured, among which Battus norvegicus was the dominant group, accounting 33. 53%. Next was 18. 41% in Rattus flavipectus. There were 95 tissue slices by the direct immunof luorescence, accounting 13. 67% and among which was 47. 0% in Battus norvegicus. RT-PCR was used to amplify and detection. Partial M and S segments were sequenced. Nucleotide sequences analysis showed that the virus detected all belonged to SEO-type. They clustered with R22, L99, Hb55, Hb86, SR11viruses and displayed visible geographical concentrate phenomenon. There was no new genotype or subtype.Conclution: The major hosts of hantaviruse in different districts were Battus norvegicus and the epidemic strains in the seven disteicts of Henan were genotyped as SEO-type. Nucleotide sequsence and deduced amino acid sequsence form different rodents were highly homologous, while nucleotide mutation had also been observed. Further studies are required to explore the possible the viruse sequence mutation.