| Objective : Revealing the relativity of different dectocting duration, dosage with the toxicant of the prepared aconite roots.Techniques: We executed this experiment by using a symmetrical designed technique. We dectocted the prepared aconite roots at identical conditions with different durations of 15 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, 4 house and 6 house, accordingly with 6 times, 12 times, 24 times, 48 time, 72 times, 96 times and 120 times of clinic crude drug dosages. We randomly set the male SPF white rats into 9 groups in an SPF experimental animal barrier system with Freund's complete adjuvant modeling, dosing accordingly. On the 30th day, we took 2/3 of the femoral vein blook of the animals, and did a series tests of hematology, blood biochemistry, anatomy and historpathology. Then we put the rest animals under a 15-day reversibale observation using the same technique and indices as above. We proceeded all the outcomes by multiple regression with the symmetrical designing 1.0, analyseing the relativity between different doctocting durations, dosages and toxicant of the prepared aconite roots decocted succus.Outcomes:1. outcome after 30-day administration: (1) tumefaction of the white rats feet: The right back feet (inflammated sides) of model group and dosing group white rats appeared severe tumefaction the first day after inflammation. the feet appeared tumefaction renewedly around the 10th day. and then again around day 20. Significant difference (P<0. 001) appeared comparing the model groups with the normal groups in the third measure; According the the comparation of Dosing groups and the model groups, the prepared aconite root with different dectocting durations could alleviate the feet tumefaction of the white rats in varying degrees and can also lead to certain therapeutic action to the feed tumefaction of the whiterats suffering with the adjuvant-caused arthritis. (2) The weight difference of the white rats: There was no significant weight difference among the groups before dosing. The outcomes showed that there was no distinct weight difference between the normal groups and the model groups, yet the weights of the white rats in the dosing groups declined dramaticly comparing with the nomal groups and the model groups. (3). hemalology testing outcomes: There was no significant difference comparing model groups with normal groups;Comparing with the model groups and the nomal groups, WBC, PLT, PT and APTT of the whit rats in the dosing groups had risen and the Rbc had dropped.(4) Blood biochemistry testing: Comparing with the model groups and the nomal group, UREA, CREA, ALT, ALP, AST, CK of the whit rats in the dosing groups had dramaticly risen.(5) organ coefficient: Comparing with the normal groups and the model groups, the livers, lungs and kidneys' organ coefficient had risen in the dosing groups. (6) pathomorphology observation results: The livers of both model group and the dosing groups had low grade lipide changes, but without any inflammation, hyperplasia and necrosis changesThe lungs of both models group and the dosing groups had focal inflammation symptoms. There's no pathomorphological changes in the rest organs.2. Reversibility observed results: (1) The weight difference of the white rats: It was no statistics value comaparing among groups. (2) hematology testing outcomes: There was no distinct difference comparing among groups. (3) Blood biochemistry testing: Comparing with the model groups and the nomal group, UREA, CREA, ALT, ALP, AST, CK of the whit rats in the dosing groups had dramaticly risen. (4) organ coefficient outcome: Comparing with the normal groups and the model groups, the livers' organ coefficient had dramaticly risen in the dosing groups. (5) pathomorphology observation results: The livers of all white rats were in the normal conditions, without any changes of congest, lipide change, necrosis,... |
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