Anticancer Effect And Mechanism Of Action Long Action Research

Objective: To investigate the effect of a new Chinese herbal drug "Changdong" on tumor and its primary mechanism. Method: Five models were used to investigate the "Changdong" ' s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong" ' s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result: "Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship. "Changdong" showed a noticeable enhancive effect when it was co-administrated with other anti-tumor agents or when it was co-applied with radiotherapy. The action mechanism study showed that an apparent cytotoxic effect of "Changdong" on ten tumor strains in vitro with a quantity-effect relationship was observed. Immunity experiment showed that "Changdong" could induce proliferation of spleen lymphocyte in mice bearing tumor and could improve NK cell' s activity, whereas has no impact on IL-2' s reproduction. It was also noticed that "Changdong" could induce human liver cancer QGY' s apoptosis in vitro, and it happened mainly on G0-G1 phase. Conclusion: "Changdong" has a igtent inhibitive impact on more than one mice tumor and human tumors implanted in nude mice and its anti-tumor mechanism is related to cytotoxicity, apoptosis induction and immunity improvement. In summary, "Changdong" is an anti-tumor agent with a prospective outlook.