Rna Interference Suppression Of Tumor Cell Vascular Endothelial Growth Factor Expression

Proliferation of vascular blood vessel is a necessary process during the development and growth of normal tissue, and angiogenesis acts an important role in the course. Physically, angiogenesis does not exist in adult except wound healing and the periodically alternation of female uterus - menstruation. However, in the phase of embryo development, the process of angiogenesis is exceptionally fluent and active, and the function of angiogenesis is tightly related to the development and differentiation of the embryo, too. Pathologically, many diseases company with angiogenesis. In the course of tumorgenesis, angiogenesis is proved to be one of the most important factor. In 1980s, Dr. Folkman first carried out the conception of angiogenesis, he and his colleagues also discovered many angiogenic factors through decades of efforts, and they first elucidate the pivotal role of angiogenesis in the development of tumor. During the course of growth and development of tumors, the solid tumor cells need a vast number of oxygen and nutrients. In the very beginning, oxygen and nutrients were acquired by freedom diffuse of the molecules. However, with the growth of tumor, the volume increases, it is impossible to support the consumption of a tumor just through freedom diffusion. Studies indicates that a tumor without intratumor blood supplement can not grow up to 1-2 mm. Unfortunately, tumor cells have developed a mechanism helping them acquiring more oxygen and nutrients - angiogenesis. Angiogenesis is the process of generating new capillary blood vessels. In the process, new capillaries develop from the extension of preexisting vasculature. Studies also demonstrated many tumor cells have the potential of secreting angiogenic factors, including growth factors, cytokines and other forms. And within all these angiogenic factors, vascular endothelial growth factor (VEGF) is no doubt to be the most important one. VEGF is a 23-kd polypeptide with the heparin-binding property. It is also a strong mitogen. VEGF can promote the migration and proliferation of endothelial cells. It is also and important target for many angiogenic regulators, such as hypoxia inducible factor, basic fibroblast growth factor, transforming growth factor etc. In this study, we use RNA interference to inhibit the expression of VEGF in vitro. RNA and protein levels of alternation were assayed by RT-PCR, immunostaining and ELISA. We successfully decrease the expression of VEGF to 97.45% and 99.02% in RNA and protein level at most. This indicates the potential applications in the treatment of tumors. Moreover, the in vivo experiment also shows an ideal effect of siRNA treatment of tumors (not introduce here).