Experimental Study Of The Compound Ulcers Spiritual Treatment Of Gastric Ulcer

ObjectThe gastric ulcer is a clinical frequently-occurring disease, the emergence of the gastric ulcer has influenced people's healthy and daily life seriously, the medicine of FU FANG KUI YANG LING is efficacious prescription to treat the gastric ulcer clinically. Let's probe into the mechanism of treating the gastric ulcer of FU FANG KUI YANG LING in terms of experiment now.Method1 Adopt big mouse acetic acid model of gastric ulcer, fetch SD big mouse 40 (each take half femaly and maly), divide into 4 groups at random , every groups ten. After anaesthetizing mouse and disinfecting skin, cut the abdominal muscle and open the abdominal cavity, find the department intersection of the stomach body and pylorus, enter the flat thorn of the micro-syringe among the layer of serous coat and skin 0.4-0.5mm , then pour into 20% of the glacial acetic acid after 0.05ml, form the small papule, still include the stomach in the abdominal cavity,inject penicillin sodium 30 000u in abdominal cavity at the same time so as not to infect , sew layer up and imprison the belly, the normal drinking water and eating. Irrigate stomach such as medicine one time a day continuing fifteen times, pluck eyeball and fetch the blood, fetch serum through the blood to do determine radiate immunology of hormone of stomach secretion and the tumour necrosises factor .Put the big mouse to death , take out the stomach body and cut whetting the appetite in the wall along the greater curvature , measure the ulcer with the micrometer , express the ulcer index with average of long foot-path and short foot-path of gastric ulcer according to document method .2 Adopt pylorus ligature model of gastric ulcer , fetch SD big mouse 40 (each take half femaly and maly) , divide into 4 groups at random, every group ten. Irrigate stomach such as medicine one time a day continuing five times,the 5th day later, begin to forbid the diet and not to forbid drinking the water,build model of pylorus ligature law according to Shay, cut along belly after anaesthetizing, pylorus ligature, sew layer up and imprison the belly, prohibit water 3 hours later , put the big mouse to death by the method to break cervical vertebra , open the abdominal cavity and combine the orifice of the stomach of ligature,collect the gastric juice, after rotating the gastric juice at odds with the community or the leadershiply , fetch the clear liquid , measure the amount of gastric juice. Fetch the gastric juice of 1ml and use 0.01mol/L NaOH titrimetric total acidity (mmol/L ) in the gastric juice. Calculate the discharging amount of total acid for an hour (mmol/h ) =( total acidity * gastric juice) /3h; Fetch the gastric juice of lml and put it in theglass tube separately, join the rare hydrochloric acid of 0.05mmol/L of 10ml, After numbering then examine the pepsin by the Mett law , examine hydrogen ion index of the gastric juice finally by adopting accurate PH test paper measurement.ResultMedicine of FU FANG KUI YANG LING can lower ulcer index, compare with the blank group, the heavy dose of group and positive group have significance difference very much, P<0.01; Raise the factor level that the tumour necrosises in the blood, compare with the positive group, the heavy dose of group has significance differences very much, P<0.01, the low dose of group has significance differences, P<0.05; Rise the function of hormone of stomach secretion in the blood, compare with the positive group, the heavy dose of group has significance differences very much, P<0.01,the low dose of group has significance differences, P<0.05.Medicine of FU FANG KUI YANG LING can suppress secretion of hydrochloric acid in gastric juice, compare with the blank group, the heavy dose of group have significance difference very much, P<0.01, the positive group has significance differences, P<0.05; Compare with the positive group,the heavy dose of group has significance differences very much, P<0.01. FU FANG KUI YANG LING Can reduce the total acidity of gastric juice , compare with the blank group, the heavy dose of group has significanc...