Kidney And The Formation Of Liver Cells And Their Gene Expression Profiles Of Bone Marrow

Objective: The purpose of this research is to observe the influence of nourish kidney (using Zuoguiwan) on that mouse bone marrow cells forming liver cells and gene expression profile of the course, and to study the molecular mechanism of that nourishing kidney manufacture marrow to form liver cell, and then to state scientific contents of "kidney manufacture marrow, marrow manufacture liver".Methods: The female BALB/C mice were randomly divided into nourish kidney team (treated by Zuoguiwan), complement Qi-Xue team (treated by Bazhentang) and contrast team (treated by physiological saline). After whole body irradiation with 9.0 Gy 60Co γ-rays, the female mice were injected via the tail vein with whole bone marrow cells of homogenic male mice to establish bone marrow transplantation model. Zuoguiwan, Bazhentang and physiological saline of 7g.kg-1.d-1 respectively treated three teams after bone marrow transplantation. By treating 2, 4, 6 months, all mice were sacrificed to get liver tissues. The left lobe of liver was formalin-fixed and paraffin embedded, other lobes of liver were freeze by liquid nitrogen. Fluorescence in situ hybridization (FISH) for both sex determining region of Chromosome Y (Sry) and albumin mRNA was used to detect male bone marrow derived liver cells in serial sections of liver tissue, and then calculate the rate of positive cells. The data was handled with SPSS statistics software. The difference of gene expression profile was detected by using mouse gene expression profile oligo-nucleotide chip. Total RNA of liquid nitrogen freezing tissues was extracted to synthetic cDNA probes that were hybridized with gene chip. Different expressed genes were selected by the ratio of hybridization signals, and the data was handled with Microsoft Access and Microsoft Excel.Results: We found that the positive cells rates of nourish kidney team, complement Qi-Xue team and contrast team are all promoting with the time length after bone marrow transplantation. The positive cells rate of each team at 6 months posttransplantation is clearly promoted contrasting to another time points, and the difference is significant (P<0.01). The positive cells rate of each team at the same timepoint is significantly different from the others. The rate of nourish kidney team at 2 months posttransplantation is higher than the rate of complement Qi-Xue team and contrast team, and the differences are all significant (P<0.01). The rate of nourish kidney team at 4 months posttransplantation is higher than complement Qi-Xue team, and the difference is significant (P<0.01). The rate of nourish kidney team at 6 months posttransplantation is higher than complement Qi-Xue team and contrast team, and the difference are significant (P<0.01). The results of gene expression profile chip hybridization shows differences of gene expression profile among different teams at 6 months posttransplantation. There're 865 genes different expressed when contrast team compared to normal female mouse. 447 genes are known, and there're 92 genes up regulation, 355 genes down regulation. There're 1147 genes different expressed when nourish kidney team compared to contrast team. 533 genes are known, and there're 264 genes up regulation, 269 genes down regulation.Conclusions: Nourish kidney (using Zuoguiwan) could promote that bone marrow cells forming liver cells, and the effect was clearly better than complement Qi-Xue (using Bazhentang) and contrast team. The mechanism of this process maybe that regulating the function of the whole body and improving minute environment of liver could help bone marrow cells move to liver and forming liver cells. The molecular mechanism of "kidney manufacture marrow, marrow manufacture liver" maybe that influenced the change of gene expression profile.