Sugar Capsule Models Of Rats And Part Of The Mechanism Of Action Of Insulin Resistance

At present comprehensive research indicate that Insulin Resistance (IR) is the same pathological base of many diseases , continuously studies show that the differ degree IR exist in many diseases such as Coronary heart disease , Hypertension , Pure hypercholesterolemia, Diabetes, Fatness ,Apoplexy. However, the mechanism of IR still can not clarify completely so far ,it is accepted that IR is the result from heredity factor and circumstance factor .According to its production laches ,it can be divided into three species-receptor front resistant, receptor resistant, receptor behind resistant.The treatment of TCM embody the synthesis function of multi factor multi target ,have its holistic superiority and characteristic. Actual studies show that many formula can alleviate IR , but most of them limit to observe the phenomena , deeper and aborder discuss about the mechanism of IR have not been put in practice, especially about cytological and molecular biological mechanisms. We choose the Chinese medicine prescription TANG SHEN JIAO NANG capsule to discuss its mechanism of alleviating IR, and make some former research for the sake of produce it.TANG SHEN JIAO NANG capsule ,the self-made formula ,was proved by observing in clinic and experiment in lab , it is the effective comparatively used to type 2 diabetes and early-stage diabetic nephropathy in clinic. Former experiments show that it can treat remarkably the diabetic nephropathy model rat induced by streptozotocin (50mg/kg ip) with excision single kidney, reduce model animals' fasting blood-glucose and serum cholesterol level, increase the serum insulin and serum albumin level, improve distinctly renal function: decrease Scr ,BUN , 24h urine trace albumin excretory rate, the kidney quotiety, and lighten the development of nephropathy . Meanwhile it can reduce remarkably the diabetes model rabbit's fasting blood-glucose level .Due to decrease blood-glucose ,serum albumin and increase serum insulin and serum albumin, TANG SHEN JIAO NANG capsule is confer to improve IR and resume some B-islet cells' function , we hope to discuss its mechanism by more experiments.Euglycemic hyperinsulinemic glucose clamp ( GC, 1979 ) is the Gold standard recognized international used to appraise IR in around tissue, especially in skeletal muscle.Overseas it is used in diagnose in clinic ,experiment in lab at large .We choose GC to research FFR in this experiment, approve TANG SHEN JIAO NANG capsule can alleviate IR remarkably. At the same time we discuss its mechanism through the change of skeletal muscle fiber components .The outcome shows that type 1 -fiber reduce marked in the FFR group with high sensitivity; and type 2a-fiber increasemarked in the group with low sensitivity ;For the more ,the ratio of type 1-fiber has a positive correlation with M and the ratio of type 2a -fiber has a negative correlation with M.The fact means that the change of skeletal muscle fiber components may be one of the determinant guidelines, it is reported that glucose intaking by assistant of insulin lies on two factors ,one is the ability to stimulate tissue by insulin itself to use glucose ,the other is the speed of transfer glucose to tissue, the later is charge for blood stream.. TANG SHEN JIAO NANG capsule's action to the skeletal muscle fiber components may have a relationship with increased blood flow by acting straightly to capillary, regulated the ratio of type 1-fiber and type 2a -fiber, improved sensitivity of insulin. The particular mechanism is not clear now because the experiment was lack of appraise of muscle blood scream , but it give a clue at least to show that regulation skeletal muscle fiber components is one of TANG SHEN JIAO NANG capsule' mechanism. Moreover, TANG SHEN JIAO NANG capsule can regulation skeletal muscle fiber components remarkably, but can not come back to normal ,We presume that its mechanism is due to colligate action including increase glucose usage in liver at same time ,improve fat metabolize .Therefore ,we choose leptin to research as contrast ,the resul...