Recombinant Human Growth Hormone In Vitro And In Vivo Human Gastric Cancer Cells

Objective To study effects of recombinant human growth hormone (rhGH) ongrowth of human gastric cancer cell in vitro.Methods After experiment was divided into control group, rhGH group,Oxaliplatin(L-OHP) group and rhGH+L-OHP group, cell inhibition rate, cell cycle ,cell proliferation index(PI) and DNA inhibition rate of human gastric cancer line,BGC823,were studied by cell culture, MTT assay and flow cytometry on differentconcentration of rhGH.Results there was no statistical significance between rhGH group and controlgroup or between rhGH+L-OHP one and L-OHP one (P>0.05) in all determineditems and it was not found that the cured line of cells growth rose. Cell inhibition rate and the cells detained in G0-G1 phase obviously increased, meanwhile, the cells in S phase and PI distinctly decreased and DNA inhibition rate obviously rose in rhGH+L-OHP group compared with control group or between rhGH+L-OHPgroup and rhGH group with pair comparison (P<0. 01).Conclusion In vitro rhGH does not accelerate the multiplication of human gastric cancer cells.