Fiber Optic Chemical Sensor Reign Of The Process Of Monitoring Rabbit Daunorubicin And Doxorubicin Blood Concentration,

OIiline and in-vivo monitoring in sitU the process of the COnCentalonof Daunerebicin and Adrimpin in theit's blood by fitw oPtcchemical sensorPostgTaduate Lv wenxuTutor Chen jianDepartment of Pharmacy, Xingjiaflg Medica1 University, Urueqi 830054.. 36...Abst r actIt is an attractive project to deve1op a method and usefiber optic chemica1 sensor(F0CS) to get the moreinformation for on1 ine and in--vivo monitoring the varyprocess of the concentration of drugs in bio1ogy and do someeffective work for research.This artic1e conc1udes two aspects work. The one work isto coIIlP1ete the instrument system of F0CS. We so1ved aniIIlPortant prob1em and gather the light into the s1im opticfiber Which diareter is l00 ll m. So the 1ight intensity fromthe optic fiber is severa1 thousands times than ever andenough to detect the faint f1uorescence. We deve1op thecomPuter soft that can make the operation more simP1e andautomatic. We a1so formu1ate a new so1--gel method to fix thef1uorescence substance on the body fiber. The above workprovide the foundation of the project.The other work is the supply for the F0CS system. Wese1ect the anticancer drugs Daunorubicin(DNR) andAdriaJnyc1n (ADM) as study objects. An anima1 mode1 is designedfor experiment and an optic fiber is 1nserted in therabbit's neck artery. DNR is determined by F0CS system basedon the feature fluorescence spectrum. The method shows theaverage recoveries 99. 9 -- 108. 0%, the average re1ativestandard deviations 2. 5--5. 3%, the detection limit 0. 05 llg/m1 (S/N=3). At the sarie time, the serun smp1e is determinedby simP1e f1uorimetry method as a coIIlPary way. ADM is37.determined by FOGS based on the fluorescence multiple quenching.A new sol梘el method is used to fix the fluorescence substance D?0 on the body fiber . The vary quenching means the vary of the concentration of ADM in rabbit?s blood.The method?s average recoveries 99.7挆 100. 2%, the average relative standard deviations is 2.95拁-3.77%,the detection limit is 0.057 it g/ml(S/N3).The fluorimetry method as a compary way with FOCS method. We can get the pharmacokinetie parameters through dealing with the data and the curve from FOGS.The study provide a new technique and guiding way for the wide supply of FOGS in enviroment and biomedical field.