Daidzein Derivatives With Pharmacological Effects Research

Daidzein(4', 7-dihydroxy isoflavone), one of important active components of pueraria lobato, has many biological activities. Because of itsbad solubility in fat solvent or water, its bio-utilization ratio is not good.Its application was restricted.To solve the problem and enhance the activities of daidzein, we modi-fied daidzein with chemical methods. Seven new and one known deriva-tives were gained. The structure of derivatives were identified by mordenspectroscopic methods. The new derivatives were: 3'-sodium sulfonatedaizein(L₁), 4',7-diacetyloxy isoflavone(L₃), 3-nitro daidzein(L₄),3'- sodium sulfonate - 4', 7 -dimethoxy isoflavone(L₁₀), 3',5', 8 - trini-tro daidzein(L₆), 3'- nitro - 4', 7 - dimethoxy isoflavone(L₉)and 3'- ni-tro - 4', 7 - diacetyloxy isoflavone(L₁₁). The known derivative was 4', 7 -dimethoxy daidzein (L₂).The effect of these compounds on the resistance of mice to normalpressure hypoxia was studied. The results showed that compound L₁,L₂,L₃,L₁0 had obvious effect of resistance to hypoxia. The active effect ofcompound L₁ and L₁₀ was higher speed than Daidzein.Meanwhile we studied the biological activities of compound L₁ andL₁₀. In mice subjected to histotoxic anoxia induced by KCN and NaNO₂,the survival time was prolonged by compound L₁ and L₁₀. We found thatcompound L₁ and L₁₀ could prolong the persistent time of gasp in decapitat-ed mice. We also found that compound L₁ and L₁₀ could antagonize ar-rhythmia induced by aconitine.