| Objective:Nowadays, cardiovascular and cerebrovascular diseases became the main reason of human death, the worse, and incidence of a disease increasing year by year. So the atherosclerosis which as onset foundation of the cardiovascular and cerebrovascular diseases receives more and more medical attention. Atherosclerosis have some characteristics, such as earlier onset,slow progression of symptoms, initial symptoms are not obvious, however, the development of clinical symptoms has been difficult to cure, and death, morbidity is very high, all this brought a heavy financial and psychological burden to society and the family, as a result the significance of prevention is far greater than the treatment. Only complete prevention of atherosclerosis, can really talk about the prevention of cardiovascular and cerebrovascular disease. Therefore, this project emphasizes the importance of prevention, according to a variety of literature reports, VC can prevent the atherosclerosis occurrence and development, so the emphasis on healthy eating, promote eating more fresh fruits and vegetables which rich in VC, and less fat diet; In addition, from the perspective of traditional Chinese medicine prescription medicine used to prevent the occurrence and development As summarized as follows:1 The establishment of a human small fruits and vegetables, oily greasy as lesions close to the lifestyle led to an animal model;2 Confirm that the subject of low-dose used in the long-term application of chinese herbs as the preventive effect;3 As the formation of VC confirmed the role of prevention, and promote a good way of eating habits;Methods:68 guinea pigs were divided into two groups, the first batch of 18 rats were randomly divided into three groups. Normal group:normal diet; model group: low-fat diet VC; Medicines:VC low-fat diet, and daily fed with traditional Chinese medicine; n=6, fed 14 days; the second batch of 50, Randomly divided into five groups, normal group:normal diet; model group:low fat diet VC; Medicines:VC low fat diet, and daily fed with traditional Chinese medicine; high VC groups:low-fat diet VC Feeding, and fed daily dose VC; statin group: low VC fat diet, and fed each day with pravastatin; n=10, fed 70 days;After feeding the corresponding number of days, the animals were treated with 10% chloral hydrate 3.3ml/100g body weight intraperitoneal injection anesthesia, under the conditions in the abdominal aorta from the lower abdominal blood, blood collection is completed, cut the guinea pig right atrial appendage, first with 0.9% 120ml saline perfusion, then 120ml of 10% formaldehyde solution perfusion fixed in situ, after fixation, were removed thoracic aorta, abdominal aorta, were placed in 10% formalin fixative, fixed Save. After fixation, HE staining, Masson staining, microscopy and photographed, morphology observetion. Used to determine the status of early animals;The blood taken for biochemical, blood, blood viscosity and oxidation index test, using SPSS statistical software, and the results were analyzed;Results:The first batch of animals:After 14 days of feeding, the state comparison:three groups of animals were eating the normal water, fur smooth and normal activities in a normal growing season in all groups. Among them, the Chinese group and the low blood when the VC group than in the control group of vascular wall is relatively thin, easily broken, indicating a lack of VC vascular fragility, poor flexibility. The model group than the normal weight group gained weight, the Medicines consistent with normal body weight;Blood biochemistry, blood and blood rheology comparison:model group and normal group:state of relatively similar, but the detection of target CHO, TG, LDL were significantly increased, weight increased significantly, in line with the material basis of phlegm. PLT, PCT, MPV, PDW were significantly higher, and whole blood viscosity, plasma viscosity were increased consistent with the microscopic blood stasis dialectical standards; that the model group model sputum silt each node card operability; and traditional Chinese medicine group and the model Group, CHO, TG, LDL lowering, weight reduction, MCV, PLT, MPV decreased blood viscosity and plasma viscosity were lower that to do this project used prescription medicine can improve blood phlegm stasis Phlegm; but unfortunately full of Chinese medicine and can not play a preventive role.Pathological changes showed:normal guinea pigs the main thoracic and abdominal aorta, HE staining showed:a full smooth lining, endothelial cell integrity, no cell aggregation lower endothelial cells, intimal thickening seen; in the smooth muscle layer of the nucleus was uniformly Oval, and smooth muscle longitudinal axis Traveling in the same direction; Masson stain showed:in the film layers of dense regular elastic fibers, showed concentric circles like distribution, a complete external elastic panels, smooth concentric circles like distribution.Model the main chest guinea pigs, compared with the normal abdominal aorta, HE staining showed:significant intimal thickening; in the smooth muscle layer of smooth muscle cells become larger and more round cells arranged in apparent disorder; Masson stain showed:Membrane layers of elastic fibers in the loose and disorganized, external elastic plate loose.Lord of guinea pig medicine chest and abdominal aorta compared with the model group, HE staining showed:mild intimal thickening; in the smooth muscle layer of smooth muscle cells become larger and the relative reduction of round cells arranged in disorder lessened; Masson stain showed:the membrane structure is relatively dense layers of elastic fibers, arranged in regular external elastic plate.The second batch of animals:After 70 days feeding, the state comparison:60 days before the normal activities of all groups can, normal consumption of water, fur smooth. However, the model group animals suddenly appeared at 61-64 days of hind limb paralysis on one side, then the state variation, the last days of almost no food water, to the entire group of animals drawn each time were not normal standing standing, weight loss, Hair removal, hair without any gloss, open the abdominal organs, especially kidneys that, a lot of fat around the liver, spine severely S-shaped scoliosis, bones can easily fracture. Hemostat in perfusion fixed easily broken when the heart, blood vessels easily rupture when that organization high brittleness, poor flexibility. Chinese group and the statin group was similarly changed, but the overall situation well in the model group, and the performance of close to two, but one side of hindlimb paralysis of the time there later in the model group, the state becomes worse after a few days, eating Water significantly reduced, and finally the statin group animals weight loss, hair removal, hair dull, unable to walk properly, it can barely stand, Medicines weaker than the statin group, but better than the model group. Statin group and the Chinese Medicines Board clamp in perfusion fixed tissue easily broken when the heart, blood vessels easily rupture when that organization highly brittle, less flexible. VC group of normal and high state best was normal, smooth coat; eat normal water, no weight loss performance. And has been in the two groups gradually increased weight status. Overall model group, groups, and the statin group were there at the end the paralysis of hind limbs, considering the lack of VC may be related to clinical symptoms in guinea pigs, the last few days of negative consumption in the state, weight decreased.Blood biochemistry, blood and blood rheology comparison:Compared with normal group, model group:Despite a few days after eating bad state of the body is consumed, body weight decreased significantly, but the CHO, TG, LDL was still significantly increased whole blood Viscosity, plasma viscosity was significantly higher, RDW, PLT, PCT, MPV, PDW increased still comply with the state of phlegm, but the body is more than 14 obvious change is the status of the entire group of animals, particularly poor and can not walk properly, to reduce water consumption, HGB, MCH, MCHC were significantly decreased, WBC increased significantly in the anemic state of the body, can not eat lead to malnutrition, low immunity, leukocyte infection, HGB decreased, reducing the blood to carry oxygen, Canada On the infection status, increased oxygen consumption, hypoxia caused endothelial cell injury, the subcutaneous tissue of hypoxic injury has resulted in the formation of atherosclerosis; and a large dose of VC plus the high-fat fed guinea pigs although There CHO, LDL increased, but the increase rate is much less than the model group, or even less than the statin group of Chinese medicine and large doses of VC can eliminate the high fat diet induced metabolic effects in TG, can eliminate high-fat diet on plasma Viscosity of blood does not affect the indicators, activities, food intake, normal guinea pig to fully explain the role of high VC; and Chinese medicine can decrease CHO, LDL, lower blood viscosity, lower MPV, PDW, improve Phlegm and blood stasis in the micro-indicators, and the statin group, although the reduced CHO, LDL effect of slightly less than the statin group, but the statin group, BUN, ALT, AST than the Chinese group is high, and statins could significantly reduce the HGB, the Body in anemia, indicating that the side effects of traditional Chinese medicine is less than the statin group, the incidence of long-term application development As preventive medicine is better than prescription statin drugs.Pathological changes showed:normal guinea pigs the main thoracic and abdominal aorta, HE staining showed:a full smooth lining, endothelial cell integrity, no cell aggregation lower endothelial cells, intimal thickening seen; nuclei of medial smooth muscle layer was Rules oval, and smooth muscle longitudinal axis line direction to go. Masson staining showed:in the film the green light scattered in the collagen fibers, elastic fibers in the membrane layers of dense regular, concentric circles showed the distribution of a complete external elastic panels, smooth distribution of concentric circles, layers of dense regular elastic fibers, showed concentric Rounded distribution;Model group, the Lord of guinea pig thoracic and abdominal aorta compared with the normal group, HE staining showed:significant intimae protrusions, endothelial cells see a lot of the lower cell aggregation, intimal thickening was; some cells in the nuclei of medial smooth muscle layer Rule was no longer elliptical, mostly large and round cells, and smooth muscle longitudinal axis does not go the same direction line; Masson stain showed:collagen fibers in the membrane decreased the membrane layers of disordered elastic fibers, loose; outside Elastic plate loose, occasionally broken.Chinese group and the statin group the main guinea pig thoracic and abdominal aortic performance similar to the model group, but better than the model group, HE staining showed:significant intimal thickening; There protruding medial smooth muscle layer of smooth muscle cells become larger and round Cells are more disordered; Masson stain showed:decrease in membrane collagen fibers, elastic fibers in the membrane structure due to dense layers of loose, disorganized, external elastic plate loose; Guinea pigs with high VC chest Lord, the performance of the abdominal aorta close to the normal group, significantly better than the medicine group and the statin group.Conclusion:1 Guinea pigs lack of vitamin C can produce consistent high-fat diet feeding each node card sputum silt animal model.2 Guinea pigs and a lack of vitamin C appear to give high-fat feeding of aortic atherosclerosis in line with the pathological changes of early lesions.3 The initial establishment of human atherosclerotic lesions closer to the guinea pig model of atherosclerosis.4 The initial recognition of Traditional Chinese Medicines in the sputum of the subject of silt in each node to improve the role of certificates.5 Emphasizing a healthy diet repeat the importance of vitamin C, advocate eating more fruits and vegetables means eating less fat. |
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