The Levocarnitine Myocardial Protective Effect Of Atp-sensitive Potassium Channels

Objective:To observe the protective effects of L-CN ischemic treatment on myocardial ischemia reperfusion injury of rats and investigate the relationship between L-CN and ATP sensitive K+ channels(KATP channels)Methods: 30 healthy SD rats were randomly divided into three groups (10 each group), ischemia-reperfusion group (I/R group), L-carnitine treatment group (L-CN group), Glibenclamide & L-carnitine treatment group (Gli+L-CN group),10 rats in each group. All the rats were subjected to left anterior descending coronary (LAD) occlusion for 30 min,followed by reperfusion for 2h. In I/R group, after 5 -minute MI,the intravenous infusion of saline to the end of the experiment, speed was 0.5ml/min; In L-CN group, after 5 -minute MI, adding 3.0g L-carnitine to 250ml normal saline infusion to the end of the experiment; In Gli+L-CN group,after 5 -minute MI, adding 3.0g L-carnitine to 250ml normal saline at the same time adding 10μmol / L of glibenclamide infusion till the end of the experiment. Each group was collected 3ml arterial blood at the end of reperfusion, and centrifugate it for 10min at 2000r/min. Then take supernatant to measure cTnI by ELISA, to mensurate the activity of serum CK and LDH with the colorimetric. Obtained apical myocardium would be fixed by 10% formalin, then was embedded by paraffin, which was to observe myocardial damage in the optical microscopy.Results: Compared with I/R group,the quantity of cTnI,LDH and CK in the L-CN group were greatly reduced (P<0.05);Compared with L-CN group,the quantity of cTnI,LDH and CK in the Gli+L-CN group were significantly improved(P<0.05).Conclsion: L-CN could protect myocardial cells, which may be due to it's effect on the ATP sensitive KATP channels.