Experimental Study Of The Anti-metabolic Syndrome In Drug Screening And The Role Of Da Anti-metabolic Syndrome And The Mechanism Of Action

Four-week-old male C57BL/6J mice were induced by high fat diet (contains 60% fat,14% protein, and 26% carbohydrate, with total energy content of 21.0 kJ/g) for more than 8 weeks. The IRF model mice characterized with insulin resistance, obesity and dyslipidemia were chosen by insulin tolerance test and used for screening anti-metabolic syndrome drugs. The Insulin sensitivity was evaluated by oral glucose tolerance test and insulin tolerance test. The levels of blood glucose, total cholesterol and triglyceride were measured by colorimetric method. Meanwhile, the body weight was monitored. As a result, some leads, including DA, were found from 23 extracts of traditional Chinese medicine. DA, an extract of traditional Chinese medicine, increased the insulin sensitivity, lowered the body weight, decreased the levels of total cholesterol and triglyceride in IRF mice. For these new functions of DA, the patent has been applied*. Further studies showed that DA improved the lipid metabolism and reduced the ectopic fat accumulation in skeletal muscle in IRF mice. The investigation about its mechanism is going on.