| Part I The isolation, culturing and identity of multi- differentiation of rat bone marrow mesenchymal stem cellsObjective :To culture rat bone marrow mesenchymal stem cells (BM-MSCs) with high purity and potential of multi-differentiation available for further study.Methods : we isolated and cultured BM-MSCs from rat with whole bone marrow adherent cultivation ,observed the morphology and characteristics of cultured cells ,identified the surface marker of cells including CD34 ,CD44 ,CD45 and CD90 by flow cytometry, induced the BM-MSCs differentiation into osteoblasts, adipocytes and cardiomyocytes.Results: Cultured BM-MSCs showed spine-like shape, positive for CD44(98.81%)and CD90(99.18%)while negative for CD34(1.49%)and CD45(9.84%). The cells were successfully differentiated into osteoblasts, adipocytes and cardiomyocytes under some special circumstances.Conclusions: Whole bone marrow adherent culture is a simple and easy operated protocol to acquire BM-MSCs with high purity and potential of multi-differentiation, enough BMSCs was obtained for furthermore research.Part II Effects of bone marrow mesenchymal stem cells transplantation on cardiac function in rats with acute myocardial infarctionObjective To investigate the effect of bone marrow mesenchymal stem cells(BM-MSCs)transplantation on cardiac function in rat with acute myocardial infarction.Methods Acute myocardial infarction was created by left anterior descending artery ligature in SD rats. Rats were divided into 3 groups: control group(only thoracotomy) ,infarction group(infarction plus saline injection) and intervention group (infarction plus DiI Labeled BM-MSCs transplantation). The left ventricular end diastolic diameter (LVEDD), left ventricular end systolic diameter(LVESD), left ventricular ejection fraction(LVEF) and fractional shortening(FS) was evaluated by two-dimensional ultrasound four weeks later.Results Compared with the control group, LVESD(3.33±0.38 vs 7.26±1.05 vs 4.49±0.53, p<0.05, respectively) in the infarction group and intervention group were significantly greater while LVEF(82.18±3.34 vs 43.32±6.63 vs 69.46±7.81, p<0.05, respectively) and FS(45.71±3.46 vs 18.76±3.39 vs 34.84±5.94, p<0.05, respectively) were reduced (p<0.05), and LVEDD(6.18±0.36 vs 8.94±1.21, p<0.05, respectively) in the infarction group was increased(p<0.05). Compared with the infarction group, LVEDD(8.94±1.21 vs 6.86±0.34, p<0.05, respectively) and LVESD (7.26±1.05 vs 4.49±0.53, p<0.05, respectively)in the intervention group were significantly decreased, whereas LVEF(43.32±6.63 vs 69.46±7.81, p<0.05) and FS(18.76±3.39 vs 34.84±5.94, p<0.05, respectively) were increased significantly. Conclusions BM-MSCs can partially improve cardiac function.PartⅢEffects of bone marrow mesenchymal stem cells transplantation on ventricular arrhythmias in rats with acute myocardial infarction Objective To investigate the effect of bone marrow mesenchymal stem cells(BM-MSCs)transplantation on inducability of ventricular arrhythmias(VA) in rat with acute myocardial infarction. Methods Acute myocardial infarction was created by left anterior descending artery ligature in SD rats. Rats were divided into 3 groups: control group(only thoracotomy) ,infarction group(infarction plus saline injection) and intervention group (infarction plus DiI Labeled BM-MSCs transplantation). ventricular arrhythmias was induced by programmed electrical stimulation four weeks later.Results Compared with the control group, the frequency of sustained ventricular tachycardia (SVT) (4 vs 26 vs 12, p<0.05, respectively) and the occurrence rate of ventricular fibrillation (VF) degenerated from VT(0 vs 5 vs 4, p<0.05, respectively) and death of VF(0 vs 4 vs 4, p<0.05, respectively) was remarkably increased in the infarction group and intervention group, and the rate of induced SVT (66.7% vs 16.7%, p<0.05, respectively)was higher in the infarction than that in the control group. Compared with the infarction group, the frequency of SVT (16 vs 8, p<0.05, respectively)and non-sustained ventricular tachycardia (NSVT) (12 vs 26, p<0.05, respectively) in the intervention group was less than that in the infarction group, but there was no significant difference in the occurrence rate of SVT/NSVT, VF degenerated from VT and death of VF between the intervention group and the infarction group (p < 0.05).Conclusions BM-MSCs can reduce the occurrence of ventricular arrhythmias events in rats with acute myocardial infarction. |
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