Establishment Of An Estrogen Receptor Based Drug Screening Cell Model For Discovering Anti-osteoporosis Drug

AIM: According to the mechanism of estrogenic sample compounds combining estrogen receptor mediated by target gene transcription and expression to express bioactivity. A reporter gene-based in vitro recombinant cell assays for detection of phytoestrogen was developed in my study to be one of the identified assays and looking for novel anti-osteoporosis drugs. METHODS: A plasmid called pERE-luc which contained the human estrogen response element was constructed. The plasmid was transfected into MG63 cell transiently. The high throughput screen model was established when the plasmid was transfected into MG63 cell stably. Samples were screened by the cell model testing the luciferase activity to estimate the hormonal activity, and E2 was positive control. Result: The Firefly lucferase activity of pERE-luc transfected cells responded well to E2, but there were no response of cells without pERE-luc to E2. The model was established Successfully and several phytoestrogens were found. CONCLUSIONS: It is indicated that the model is convenient, fast, stable, effective, trace and simple.