The Experimental Study Of Building Spinal Tuberculosis Models In New Zealand White Rabbits

Objective: Spinal tuberculose models were built by inserting standard tuberculose strain of H37Rv into the upper edge of lumbar 5 in New Zealand white rabbits, then to supply animal model for substructure investigation of bone tuberculosis. Methods: The models were built through drilling hole on the 5th anodic lumbar vertebrae in unsensitized New Zealand white rabbits, and the hole was inserted which filling gelatin sponge and adsorbed MTB suspension (bacterium quantity: 0.5mg/0.1ml), NS control and vacant group was set up at the same time, which were confirmed though general overview, images, histopathology and bacteriology postoperatively. Results: After being infected by standard tuberculose strain of H37Rv on the lumbar spine in New Zealand white rabbits, local reaction was fairly conspicuous, systematic reaction was fairly light. Nine rabbits had been completed experiment in infected group (9/12), 3 rabbits of them appeared athrepsy and bad foodintake(3/9), 2 masculine rabbits of them appeared in X-ray films one month postoperatively(2/9), There were 4 masculine rabbits in X-ray films(4/9), 6 masculine rabbits in CT films(6/9), and 7 masculine rabbits in MRI films(7/9)two months postoperatively. The HE coloretur of body of vertebra and latero-vertebra soft tissue shew pus cell or epithelioid cell or cellular necrosis focus in infected group, 60.0 percents of liquor puris of rabbits shew mycobacterium tuberculosis grown. The achievement ratio of modelling was 66.7%(6/9). Conclusion: Injected suitable dosage of standard tuberculose strain of H37Rv into the centra of spinal column in unsensitized rabbits, the rabbit models of spinal tuberculosis were established susscessfully which were pathologically similar to spinal tuberculosis in humans. The models were followed up and confirmed successfully by symptoms, images, histopathology and bacteriology.