Studies Of Eicosapentaenoic Acid And Gemcitabien On A-549 Human Lung Cancer Apoptosis

OBJECTIVE To observe the effects of anti-proliferation and inducing apoptosis on A-549 human lung cancer cell line induced by EPA and its synergistic effect with Gemcitabien, in clinical for reduce GEM then dosagedrug toxicity provide experimentMETHODS MTT assay was used to determine the effect of EPA and Gemcitabien on A-549 human lung cancer cells growth inhibitory. Use inverted microscope HE staining and AO/EB double staining detection A-549 human lung cancer cells apoptosis FCM was used to detect cell cycle distribution of A-549 cells and apoptotic rate.RESULTSâ‘ The MTT test showed that A-549 cells exposure to EPA with Gemcitabien Gemzar for A-549 grow inhibition at EPA combined with Cisplatin shows significant synergistic anti-tumor effect on A-549 cells.â‘¡Morphology survey inverted microscope. survey The A-549 human lung cancer cells with EPA showed cellular volume small and round,nuclear chromatin condensation, loose between the cells and adherent ability decrease under invert microscope. Human A-549 lung cancer cells stained by AO/EB double fluorescent staining were observed under fluorescent microscope, appearing as chromation orange-red condensation in nuclear, orange-red or orange-yellow fluorescence disappeared and light green fluorescent apoptotic bodies around cells after induction of EPA.â‘¢FCM survey EPA can A-549 cells apoptosis; some typical sub-dipioid peaks were observed, the percentage of cells in S phase increased and the percentage of cells in G0/G1 and G2/M phase decreased after the cells treated by active components of menispermum dauricum; the active components of menispermum dauricum could arrest A-549cells in G0/G1 phase;CONCLUSIONS1 EPA has inhibition of proliferation and inducing apoptosis for A-549 cells in vitro; and the effect was dose-and time-dependent in experiment range, compared with control2 EPA with Gemcitabien Gemzar shows significant synergistic anti-tumor effect on A-549 human lung cancer cell line.3 EPA lead A-549 cell apoptosis apoptosis effect may be connected with S phase...