Expression Of Mmp9, Vegf, Cd34 And Nm23 In Human Glioma And Its Relationship

Invasive behavior of tumor malignant has extremely close relationship to the extracellular matrix.Tumor cells secret a variety of proteolytic enzymes such as matrix metalloproteinase(MMP),serine proteinase(urokinase,plasminogen) and cysteamine ammonia Acid,and so on,so as to get through the channel for the invasion and metastasis of cancer cells.MMP9 is an important matrix metalloproteinases,and playes an important role in the process of extracellular matrix degradation and invasion of the peripheral tissues,at the same time impacts the formation of tumor angiogenesis as the members of starting system.Foreign study found that the activation and(or) overexpression of MMP 9 have a good correlation withmetastasis and(or) degree of malignancy,in malignant melanoma,endometrial cancer,prostate cancer and primary brain tumors and other tumors,and can serve as prognostic markers.Growth,invasion and metastasis of malignant tumor dependent on angiogenesis,Vascular endothelial growth factor(VEGF) is the specific type of vascular endothelial cell stimulating factor,the main biological function of which is to increase vascular permeability,induce endothelial cell to express proteolytic enzymes, and promote proliferation of vascular endothelial cell and angiogenesis.CD34 is a new kind of marker of microvascular,it can show vascular endothelial with high specificity,and may become the preferred marker,nm23 is a new tumor suppressor genes,which is closely related with tumor invasion and metastasis.It affects cell differentiation and biological activity of cytoskeleton protein through cell signaling reactions of G-protein-mediated regulation,and thus inhibits tumor infiltration and metastasis.It is general considered that its downexpression have correlation with degree of malignancy,metastasis and poor prognosis in a variety of tumor.Incidence of glioma is high,and postoperation it easily reoccurs.The key of the poor prognosis lies in its vicious attacks.So an integrated in-depth study to the proliferation and invasiveness of malignant brain tumor will help us to improve the level of its diagnosis and treatment,which has become topical issues participated by scholars at home and abroad.There are few domestic reports about the expression of MMP9,VEGF,CD34 and nm23 and its relationship in glioma as yet.THIS study detected MMP9,VEGF,nm23 and CD34 protein expression in 50 cases of glioma,30 cases of normal brain by immunohistochemistry,and explored their mutual relations. These might provides theoretical basis for further exploring the mechanisms of development of glioma and finding effective measure for blocking invasion and metastasis.Methods1.Using immunohistochemical SP to detect MMP9,VEGF,nm23 protein expression in the 50 cases of cerebral gliomas,30 cases of normal brain tissue respectively, and count microvascular density(MVD) according to CD34 immunohistochemical staining.2.Statistical analysis:All the dates were analyzed by SPSS 11.0 statistical package. The comparison of positive rates were usedχ2 test(Chi-square);the measurement data between the two groups using t-test;the relationship between the two variables were analyzed by correlation analysis.Size of test is a=0.05Results1.Immunohistochemical results show that positive rate of MMP9,VEGF,nm23 in glioma and normal brain tissue were 68.0%(34/50),76.0%(38/50),52.0%(26/50) and 0%(0/30),26.7%(8/30),83.3%(25/30).There was statistically significant difference between the two groups(P＜0.05).2.Immunohistochemical results show that positive rate of MMP9,VEGF,nm23 in low and high grade malignant glioma were 52.2%(12/23),60.9%(14/23), 69.6%(16/23) and 81.5%(22/27),88.9%(24/27),37.0%(10/27).There was statistically significant difference between the two groups(P＜0.05). 3.It showed that MVD values(marked by CD34) were 13±4.61 and 40±15.39 in normal brain tissue and glioma tissue,37.36±3.67 and 52.34±19.94 in low-grade and high-grade glioma.There was statistically significant difference between the two groups(P＜0.05).4.Statistical analysis showed that the expression of VEGF and MMP9 in glioma tissue were positively correlated(P＜0.05),the expression of VEGF-positive group MVD values higher than that of VEGF-negative group(P＜0.05).VEGF in plastic nm23 stromal tumor tissue was negatively correlated(P＜0.05).the expression of nm23 and VEGF in glioma tissues were negatively correlated(P＜0.05).Conclusions1.The expression of MMP9 and VEGF in human glioma tissue is higher than in normal brain tissue,and high malignancy is higher than low-grade gliomas, suggesting that their expression in glioma are related with malignant glioma progression.2.Nm23 expression in glioma is lower than in normal tissue,and in low malignant glioma is higher than high malignancy group,suggesting that lose or decreases expression of nm23 may play an important role in the invasion and proliferation in human glioma.3.CD34 expression which is clear and reliable is related with the degree of malignancy in gliomas,may become an ideal target determining glioma microvascular density.4.MMP9 and VEGF expression is correlated positively,so we speculate they may synergy in the development of glioma,nm23 and VEGF expression is correlated negatively,suggesting that the imbalance in the expression of both jointly promote the progress of malignant glioma.