Dscam In Rat Bone Marrow Mesenchymal Stem Cells To Differentiate Into Neurons Change

[Background and Objectives] Bone marrow mesenchymal stem cells due to a strong division, proliferation and self-renewal capacity, the traditional view that MSCs differentiate into muscle cells, bone cells, blood cells and other tissue cells. MSCs recent study found that under certain conditions can differentiate into nerve cells in the horizontal, such as the mitotic factor, retinoic acid compounds, neurotrophic factors, such as Chinese medicine-induced. Down syndrome cell adhesion molecule (DSCAM) gene located on 21q22, its over-expression, such as chromosome 21 of the three-body, that is, Down's syndrome, caused by nerve cell migration, proliferation and differentiation of the abnormal, leading to congenital developmental disorder smart. If DSCAM low expressing appeared to increase the abnormal nerve connections. Moreover, DSCAM among neurons connections must be needed the cell adhesion molecule, in the axonal and dendritic growth and synapse formation and the formation of the neural network plays an important role and maintain.To explore the effects of Down's syndrome cellular adhesion molecule (DSCAM) on differentiation of rat marrow mesenchymal stem cells (MSCs) into neuron in vitro.[Methods] The first step was to induce MSCs from Sprague-Dawley rat into neurons by baicalin in serum-free medium for 6 days. Further, the expression of DSCAM before and after induction were observed and evaluated by immunocytochemical staining and Western blot assay. Meanwhile, after knockdown of DSCAM by siRNA transfection, the differentiation rate of neurons derived from MSCs were also measured.[Results] Before induction, the expression of DSCAM could not be detectable on MSCs. After induction for 24h, MSCs had little expression of DSCAM (1.71 %±0.67 %); at 6 hours, the expression of DSCAM were increased (15.79%±4.24%); and the expression of DSCAM could reached the highest level at 3 days (53.16%±5.94%); then the expression of DSCAM were decreased rapidly at 6 days (28.99%±6.72%). However, after DSCAM-siRNA transfaction, the expression of DSCAM on MSCs were significantly breakdown. On the anther hand, MSCs did not express neuron-specificβ-III-tubulin before induction. After induction for 6 hours, 3 days, 6 days, the expression ofβ-III-tubulin were (1.40%±0.79%), (41.59%±3.17%), and (59.11 %±4.76 %) respectively. But after DSCAM-siRNA transfaction, the expression ofβ-III-tubulin were at significantly decreased levels (28.57%±2.91%, 43.90%±12.31%).[Conclusions] These findings indicate that DSCAM may play an important role in MSCs differentiating into neural cells.