Survivin, Bcl-2, Pcna In Breast Cancer's Expression And Significance

Background and Purpose:In the global female occurs in the malignant tumor, breast cancer is the most common malignant tumor,occupies the feminine malignant tumor the first place.In recent years breast cancer disease incidence rate assumed the trend of escalation,the morbidity age is youth oriented day by day.At present,on clinical receives a medical examination for the first time in breast cancer patient, because the early diagnosis,the judgment prognosis's biological indicator lacks, majority for intermediate stage,later period.Breast cancer designated object examination may reflect that the tumor the biology characteristic,to the auxiliary diagnosis,the instruction treatment,the judgment prognosis has the important meaning.Breast cancer's occurrence of,the development are more than stage multi-step complex processes,anti-perishes is weakly breast cancer cancer cell realizes one of the attack,the shift,multiplication committed steps.Survivin took discovered until now strongest perished weakly the inhibiting factor,its organization distribution had the obvious cell selectivity,main expression in embryo,in growth embryo organization and majority tumor organizations.Survivin has the suppressor cell to perish weakly,the promotion cell transformation and the participation cell's mitosis,blood vessel's production and functions and so on tumour cell drug resistance production.bcl-2 takes perishes weakly one of inhibiting factors,lies between the cell which through the high expression leads to perish weakly the barrier,makes the genetic material the rate of mutation to increase,thus participates in many kinds of tumors the occurrence processes.PcNA one of as cell multiplication factors,(the S time) has the close relevance with mitotic cycle's in DNA synthesis time,its expression may take breast cancer's one kind of auxiliary diagnosis target.This research plans to use the immunity histochemical method(the S-P law) to examine Survivin,Bcl-2 and PCNA,in breast cancer organizes the change characteristic.Discusses Survivin,Bcl-2 and PCNA three and breast cancer's occurrence,the development and the prognosis relevance.Increases to breast cancer multi-phased evolution molecular mechanism's understanding.Provides the important rationale and the method for the clinical breast cancer biological controls.Materials and Methods:60 example breast cancer patient specimen surgery excises the specimen from the Henan Province Zhengzhou University first affiliated hospital March,2006 to January,2007.all Is the female,the age 33～75 years old.Before the patient technique,has not accepted the chemotherapy,the radiotherapy and other treatments.All specimens after organization pathology inspection confirmation for breast cancer wetting quality drive pipe cancer.All surgeries excise the specimen to pass through 85%alcohol fixation,the paraffin wax embedding,the serial section,carries on the organization pathology and the immunity group inspection.The immunity group uses the S-P law examination breast cancer to organize Survivin,bcl-2 and the PCNA protein expression.Uses SPSS11.5 statistics software processing,each group of rate comparisons use x~2 the examination,the inspection standards takeα=0.05.Results:1.The Survivin expression is mainly located at the cytoplasma,the masculine gender symbolized that presents the yellowish brown pellet for in breast cancer cancer cell's cytoblastema,the Survivin masculine gender expression rate is 70.00%(42/60). Grades the survivin protein according to the histology,in breast cancer organizes the immune response masculine gender rate respectively is:Ⅰ+ⅡLevel 61.90% (26/42),ⅢLevel 38.10%(16/42),each group of between non-significance difference (P＞0.05).The survivin protein in≤45 year-old group's masculine expression rate is 69.23(18/26);In the＞45 year-old group's masculine expression rate is 70.59 (24/34).Breast cancer organizes the survivin protein not to have the significance in disparity in age patient masculine expression difference(P＞0.05) the survivin protein is having the partial lymph node shift group's masculine expression rate is 89.29% (25/28);Is higher than obviously has not had the partial lymph node shift group,the two have the significance difference(P＜0.01).The Survivin protein in ER,in the PR masculine gender's masculine expression rate respectively is:61.11%(22/36);76.92% (20/26).The Survivin protein immunity expression in ER,PR are cloudy,in masculine gender not remarkable difference(P＞0.05).2.The Bcl-2 expression locates in the cytoblastema either the amnion,the masculine gender symbolized that presents the yellowish brown pellet for the cytoblastema or the amnion(picture 6.7),the Bcl-2 masculine gender expression rate is 66.67% (40/60).Grades the Bcl-2 protein according to the histology,in breast cancer organizes the immune response masculine gender rate respectively is:Ⅰ+ⅡLevel 30.00%(12/40),ⅢThe level 70.00%(28/40),between two groups has the remarkable difference(P＜0.01).The Bcl-2 protein in≤45 year-old group's masculine expression rate is 65.38(17/26);In the＞45 year-old group's masculine expression rate is 67.65 (23/34).Breast cancer organizes the Bcl-2 protein not to have the significance in disparity in age patient masculine expression difference(P＞0.05).The Bcl-2 protein is having the partial lymph node shift group's masculine expression rate is 82.14% (23/28);Is higher than obviously has not had the partial lymph node shift group,the two have the significance difference(P＜0.05).The Bcl-2 protein in ER,in the PR masculine gender's masculine expression rate respectively is:61.11%(22/36);73.08% (19/26).Bcl-2 protein immunity expression in ER,PR not remarkable difference (P＞0.05).3.In breast cancer organizes the Survivin protein expression and the Bcl-2 protein expression has the remarkable relevance(P＜0.01).The PCNA masculine gender expression is mainly located at the tumor cell nucleus,the cytoblastema obviously weak masculine gender,the masculine expression rate is 71.67%(43/60).Survivin the protein expression masculine breast cancer PCNA masculine gender is higher than Survivin obviously protein expression negative mammary gland cancer cell PCNA, between the two difference has statistics significance(P＜0.01).Bcl-2 the protein expression masculine breast cancer PCNA masculine gender is higher than Bcl-2 obviously protein expression negative mammary gland cancer cell PCNA,between the two difference has statistics significance(P＜0.01).Conclusions:1.Survivin,Bcl-2 protein,in breast cancer organizes the masculine expression rate to be high,indicated that the two participated in breast cancer's occurrence,the developing process.The two in have breast cancer which the lymph node shifts to organize the masculine expression rate to be higher than the non-lymph node shift breast cancer organization,prompts Survivin and Bcl-2 possibly is judges breast cancer prognosis the important designated object.The two in breast cancer organizes the expression to have the obvious relevance,prompts Survivin and the Bcl-2 common expression in the occurrence which,the development,the infiltration,the shift organizes at breast cancer is possibly playing the important synergism.2.Examines Survivin,Bcl-2 and PCNA jointly,in breast cancer organizes the expression possibly to play the influential role in the judgment breast cancer prognosis aspect.