Effects And Mechanisms Of Pparγ On The Proliferation And Differentiation Of Rat Osteoblasts In Vitro

Objective To study the effect of rosiglitazone, the agonist of peroxisome proliferator activated receptorγ(PPARγ) on the proliferation and differentiation of osteoblasts. To determine the possible pathway of rosiglitazone.Methods After cell culturing identify the cells by ALP staining and mineralized nodules. MTT assay and PNPP assay were used to observe the effects of different concentrations of rosiglitazone on proliferation and differentiation of the cell. RT-PCR detect the expression of CTGFmRNA on TGF-β1 induced obsteoblast after affected by different concentrations of rosiglitazone.Result (1)Different concentrations of rosiglitazone can not change markedly the proliferation of osteoblasts.(p>0.05) (2)Compared with control group, all different concentrations of rosiglitazone can suppress ALP activity in osteoblasts. (p<0.01) (3)Rosiglitazone suppress the CTGF mRNA expression of TGF-β1 induced osteoblats in a dose-dependent manner. (p<0.01)Conclusion In vitro, rosiglitazone can inhibit the CTGF mRNA expression of TGF-β1 induced rat osteoblasts. And we can infer that rosiglitazone may play a potential role in preventing the differentiation of the rat osteoblasts.